Question
What is the purpose of the Ames test? How are his- bacteria used in this test?
What is the purpose of the Ames test? How are his- bacteria used in this test?
Answers
Ames test is a method of assaying the mutagenic ability of chemical compounds. This test is based on the ability of reverse mutation or back mutation.
In Ames test a strain of bacteria is used particularly Salmonella typhimurium having mutation in gene responsible for histidine synthesis. These auxotrophs are his- and require the presence of histidine in the growth medium. His- strain is cultured in the presence of chemical to be assayed to observe the effect of the chemical on the bacterial ability to resynthesise the histidine. If the chemical is able to induce reverse mutation in the bacteria so that it can synthesise histidine then this chemical is a potential mutagen.
Similar Solved Questions
1 answers
Pre-Laboratory Assignment 1. What safety precautions must be observed when using (a) pyridinium tribromide? (b) acetic...
Pre-Laboratory Assignment 1. What safety precautions must be observed when using (a) pyridinium tribromide? (b) acetic acid? 2. Calculate the theoretical yield for the bromination of both stilbenes and cinamic acid, assuming the presence of excess pyridinium tribromide. Note the theoretical yields h...
1 answers
What is the bond order of N2 gas? It's molecular orbitals have ten 18) bonding orbital...
What is the bond order of N2 gas? It's molecular orbitals have ten 18) bonding orbital electrons and four antibonding orbital electrons....
1 answers
Complex anaylsis, cite all theorems used. Y are one Consider the real valued function ulx,y) with...
Complex anaylsis, cite all theorems used. Y are one Consider the real valued function ulx,y) with x and real variables. For each definition of ucxy) below, find whether there cette exists real-valued function v(x,y) such that f(2)= u(x, y) tivcx,y) is a function analytic in some DEC. If such such v(...
1 answers
The Labor Bureau wants to estimate, at a 90% confidence level, the proportion of all households...
The Labor Bureau wants to estimate, at a 90% confidence level, the proportion of all households that receive welfare. The most conservative estimate of the sample size that would limit the margin of error to be within 0.034 of the population proportion is:...
1 answers
What should she be prescribed? Sherry is a 30-year-old woman who presents with complaints of a...
what should she be prescribed? Sherry is a 30-year-old woman who presents with complaints of a yeast infection that keeps coming back. She also reports increased thirst and urinary frequency. She has self-treated her yeast infection and says that those symptoms have cleared at the present time. Over...
1 answers
A 17.0 kg monkey hangs from a cord suspended from the ceiling of an elevator. The cord can withstand a tension of 220 N and breaks as the elevator accelerates. What was the elevator minimum acceleration (magnitude and direction)?
A 17.0 kg monkey hangs from a cord suspended from the ceiling of an elevator. The cord can withstand a tension of 220 N and breaks as the elevator accelerates. What was the elevator minimum acceleration (magnitude and direction)?...
1 answers
HC,H3O2(aq) + H20() 근 H,O+(aq) + C2H30,-(aq) hedissaciation of ethanoic acid, HC,H,0,(aq), is represented above. A...
HC,H3O2(aq) + H20() 근 H,O+(aq) + C2H30,-(aq) hedissaciation of ethanoic acid, HC,H,0,(aq), is represented above. A student is given the task of determining the value of K, for HC H,O(aa) using two different experimental procedures (a) The student is first asked to prepare 100.0 ml of 0.115 M ...
1 answers
1. What is the pH of a 0.35 mol/L solution of acetylsalicylic acid (C9H804)?
1. What is the pH of a 0.35 mol/L solution of acetylsalicylic acid (C9H804)?...
1 answers
The temperature-dependent form of the extended Debye-Huckel equation is where γ is the activity coefficient, ε...
The temperature-dependent form of the extended Debye-Huckel equation is where γ is the activity coefficient, ε is the dielectric constant of water (a unitless quantity), T is the temperature (in units of K), z is the charge of the ion, μ is the ionic strength (in units of mol/L), an...
1 answers
Problem1 Part 1: An asset having a six-year useful life and a salvage value of $3,000...
Problem1 Part 1: An asset having a six-year useful life and a salvage value of $3,000 was acquired for $21,000 cash on January 1. The company estimates the asset will produce 60,000 units over its useful life. During the year the asset was used to produce 14,020 units. Using straight-line depreciati...
1 answers
Please prove?
In the figure below ABCE is a paralleogram. F and G are an points on CE and EB respectively such that FG is parallel to CB. Prove that area of #DeltaACF=DeltaABG#...
1 answers
Brief Exercise 16-3 Weber Company purchases $52,600 of raw materials on account, and it incurs $65,800...
Brief Exercise 16-3 Weber Company purchases $52,600 of raw materials on account, and it incurs $65,800 of factory labor costs. Supporting records show that (a) the Assembly Department used $26,100 of raw materials and $40,700 of the factory labor, and (b) the Finishing Department used the remainder....
1 answers
George Caloz & Frères, located in Grenchen, Switzerland, makes luxury custom watches in small lots. One...
George Caloz & Frères, located in Grenchen, Switzerland, makes luxury custom watches in small lots. One of the company’s products, a platinum diving watch, goes through an etching process. The company has recorded etching costs as follows over the last six weeks: Week Units Etching ...
1 answers
1. (a) Name the symmetry groups of the following two planar shapes, and state in each case the or...
1. (a) Name the symmetry groups of the following two planar shapes, and state in each case the order of the group and whether the group is Abelian. 1. (a) Name the symmetry groups of the following two planar shapes, and state in each case the order of the group and whether the group is Abelian....
1 answers
4- How many gram of Magnesium is in 5.6 gram of Mg(OH)2?
4- How many gram of Magnesium is in 5.6 gram of Mg(OH)2?...
1 answers
Included proper units when HLLL 1. The following kinetic data were generated for the enzyme SpB1,...
included proper units when HLLL 1. The following kinetic data were generated for the enzyme SpB1, this enzyme has an unknown biological function. Using the information provided below answer questions la-le. All reactions had 0.1 mg/mL SPBT which based on amino acid sequence and gel filtration column...
1 answers
Question 13 of 16 ) Given the reactions, X(8) + 0,(8) + XO(5) AH = -581.3...
Question 13 of 16 ) Given the reactions, X(8) + 0,(8) + XO(5) AH = -581.3 LJ XCO, (a) —- XO(s) +CO,(6) AH = +225.5 kJ what is all for this reaction? X() +20,(8) + CO2(e) + XCO,(3) AH- MacBook Pro Q Search or enter website name Válå š â ; ló M ? s ? ho command o...