Question
If given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your...
If given a 1.0 kbp human genome fragment that does not contain any repeated sequences. Your plan is to use it as a probe for a Southern Blot containing your friend's genomic DNA. You prepare your gel by digesting with the following restriction enzymes in the following lanes:
Lane 1: size marker
Lane 2: EcoRI
Lane 3: HindIII
Lane 4: BamHI
Your friend happens to be homozygous for the region you are probing. Does that mean you will only see one band in each lane?
Question:1 - Concerning the information above, what if you decide to conduct the same procedure on a different friend who happens to be heterozygous for the region being probed? Would you expect to see only one band per lane? Explain detailly
please answer question in Bold (1) with respect to information above.
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