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WCr IneTollowing questions Answers shoulc Enzyme Km(M) kcat(s-1) 9.5 x 10-5 1.4 * 104 2.5 x 10-2 1.0 * 107 5.0 * 10-6 8.0 x 102 has the highest affnity for suhstret...

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WCr IneTollowing questions Answers shoulc Enzyme Km(M) kcat(s-1) 9.5 x 10-5 1.4 * 104 2.5 x 10-2 1.0 * 107 5.0 * 10-6 8.0 x 102 has the highest affnity for suhstrete>

WCr Ine Tollowing questions Answers shoulc Enzyme Km(M) kcat(s-1) 9.5 x 10-5 1.4 * 104 2.5 x 10-2 1.0 * 107 5.0 * 10-6 8.0 x 102 has the highest affnity for suhstrete>



Answers

The $K_{M}$ for the reaction of chymotrypsin with $N$ -acetylvaline ethyl ester is $8.8 \times 10^{-2} \mathrm{M}$, and the $K_{M}$ for the reaction of chymotrypsin with $N$ -acetyltyrosine ethyl ester is $6.6 \times 10^{-4} \mathrm{M}$. (a) Which substrate has the higher apparent affinity for the enzyme? (b) Which substrate is likely to give a higher value for $V_{\max } ?$

Okay, So, uh, this prom 23 is sort of about the enzymes that are happening in the beginning of glycol Assis. Which course starts with a glucose, right? And then the first thing that's gonna help in our sort of step one is that the hex of tennis? Thanks. So kinase is going to phosphor allies that race was gonna sort of prepare this glucose, and we end up with our glucose with six carbon and the phosphor relation. Okay. And some glucose. Six fast feet, then. Ah, kids sort of buy by the fast for glucose Osama race when she becomes our fruit to six. Fast, mate. Okay, so they get our fruit too. Six fast eight on it is the fruit to six phosphate. It's the color we want is that one, um, breaks up into the true toes. One by phosphate scenario the fruit just one by phosphate. In this stage, we're going to see another enzyme. And this one is thief Ospel Fruit do kidneys, which is a mouthful. Says the fuss. Oh, fruit. Uh oh. Kin nace go the side of it here. Okay. So that the front the fastco fruit do kidneys actually plays a sort of regulatory step here. Okay, it It sort of has this feature where if there are high levels of a teepee or it's a treat, or if we have a low pH, it's going to reduce. It's, uh, Catalan is that it's gonna then reduce the enzymes activity, right? So they're sort of responding to its environment, and in that way it actually becomes the sort of main enzyme control in Glad Collis. All right, many of the other enzymes, like the hex of Connex. They're sort of just catalyzing a reaction by, just in this case, the phosphor relation. But with the possible food, in case it's actually, uh two observing the environment and then responding. So the answer for which and them hazardous control, there's going to be a little choice. B

Action here wants us to calculate the Valley of K M for the reaction. So it's going to be 2.86 times 10 to apartment two moles.

In a paradox Chemical reaction reducing agent is an element or compound that loses electron mhm and itself get oxidized. Right oxidizing agent is a compound that accepts the electron and dad reduced the electron affinities can be measured by measuring the dogs potential. Mhm. That is the potential value when the reduction and oxidation of a certain battle or chemicals are in balance with each other. And it is measured in rules the pair of compound that have the most negative electrons. Redox potential have the weakest affinity for electron and therefore contain a carrier with the strongest tendency to donate electron. Okay. And conversely, those pairs of compounds that have the most positive dogs potential have the strongest affinity for electron and hands contain carrier with strongest tendency to accept electron. So in the given cushion. Mhm the first answer is the strongest reducing agent is an A beach. Having the value of redox potential Is -0.32 Vault. Second answer. The strongest oxidizing agent is oxygen that have the the dogs potential value Plus 0.816 World. That is the highest value in the given list. And the third answer is strongest oxidizing agent. As we have seen, that strongest oxidizing agent has highest electron affinity, so oxygen has the strongest electron definitely.

So going back to our so going back to our lock and key mechanism, where substrate act as a lock and enzyme acts as a key substrate. An enzyme attach together in such a way that the part of the substrate where the reaction is to occur occupies the active site of the anxiety. After the reaction occurs, the products are liberated, so when separated enzyme react, they form enzyme substrate complex, and after the work has been performed by design, it leads to formation of Rhonda and the deep attachment of anxiety. So the new molecule acted on buyer enzyme are called as substrate.


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