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###### LhieenehraletSou UtinahaNneAattFlt uEcWM-LEtnn {ElalneslchestnlnInkork Mea uneenaaranaLStep IL: De-pruteinizationTransfer 50 ml of filtratc into clean 250 ml conical flask.10. Add 10 ml chloroform into the conical flask You should sce layer of chloroform scparated from the homogenate at the boltomthe flask:contenle of the flask few limncs- DO NOT M. Gently swirl theVIGOROUSLY MIX SOLUTION:What happens this stage4nts

Lhieene hralet Sou Utina ha NneAatt Flt uEc WM-LEtnn {Elalnesl chestnln Inkork Mea uneenaaranaL Step IL: De-pruteinization Transfer 50 ml of filtratc into clean 250 ml conical flask. 10. Add 10 ml chloroform into the conical flask You should sce layer of chloroform scparated from the homogenate at the boltom the flask: contenle of the flask few limncs- DO NOT M. Gently swirl the VIGOROUSLY MIX SOLUTION: What happens this stage 4nts

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