You are performing the Ames mutagen test on four different chemicals, A, B, C, D. Using the table below predict the carcinogen test_Mutagen test 10-3 Carcinogen tes...

The Ames test a. provides a way to determine if any type of cell has experienced a mutation. b. provides a way to determine if an agent is a mutagen. c. allows researchers to experimentally disrupt gene activity by causing a mutation in a specific gene. d. provides a way to repair mutations in bacterial cells. e. does all of the above.

You hear? Asked what type of mutations, if any, do that three passive hides induce. So essentially, we know that and Am's test, which is a S test, can be used to determine the beauty Jenness ity off chemical soap from the given off results. People would just founded this image front of this particular chapter. We know that pesticide wanting calls his transition mutations, which are mutations going from periods appearing or permitting to permitting. So this passage that could be neutralised by liver enzymes and cannot cause friendship mutations. On the other hand, P two does not cause transition mutations. Therefore, liver enzymes will conferred into a frame shift. Um, I need to do past, said three causes of transition element mutinous mutations rather, and it is going to be unaffected by a liver enzymes, and because of this, it cannot cause from ship mutations.

This picture shows the Ames test, which is used to identify muted Jin's. It uses mutant salmonella bacteria, which is a history, and occid Roaf to mix with the substance that is suspected to be a muted gin and a liver extract.

So we're continuing on with our DNA topic on. We'll jump straight into the content here because we've already looked at some background basic information. So the first thing we're taking look at here is purified water. So the play is treated with this purified water that acted as the negative control in this given experiment. Because the water is not a muted gin, so it will not result in any sort of reversion, and therefore the number off colonies observed will be, in fact, the minimum number on represent false positives. So all of the mutations must be compared with the negative control plate before we start to interpret results. The second part we have are known Musa Jen. So the plate containing the known mutation acts as our positive control in the experiment. So, um, you to general caused the reversion on the growth of colonies on the play itself, which will provide a basis for comparison off unknowns that we've tested. Sad part we have is the experimental sample to the experimental sample. The sample that's tested shows a very small amount off growth of colonies, and this is similar to the water negative control play that we discussed in the first part, so this indicates the absence off the reversion upon treatment off salmonella with the compound. Therefore, this test to compound seems to like the youth and the Genesee. So any final part we have to consider the predominant function of the liver is the metabolism off toxic molecules in the body as well as other different molecules by its enzymes. So these metabolic reactions tend to result in the detoxification off compounds, which makes them harmless to the body. However, in some cases the metabolic reaction makes a non toxic compound toxic, which would not be a good thing. So, for example, in the Ames experiment, the chemical tested in place, see was retested after the treatment with liver ha Mancini on, we see a large number of colonies observed on the play itself. This indicates that the extensive reversion it induced by the chemical of the process in the presence off the liver homogeneous upon the plate. So this is indicative of the fact that the chemical, upon treatment with the liver homogeneous eight, was converted into a very mutiny genic compound. So this is the opposite of the sort of reaction that would be looking for the liver to carry out. So a comparison of this plate where the positive controlled also further supports the idea that we do have a mutiny genic compound upon the plate now.

This question asks. Which of the following regarding the Ames test is true. We have. It is used to identify newly formed Aqsa Traffic mutants. It is used to identify meetings with restored bio synthetic activity. It has used identify spontaneous mutants where it is used to identify mutants lacking photo reaction, reactivation activity. So we know that based on our answer choices, choice be would be the correct answer, because the Ames test will be used to identify Mulan's with that restored bio synthetic activity. Because that is the purpose behind the abscess. And the aim senses just looking for that restored bio synthetic activity of our mutants. So choice. Bizarre, correct answer.