## Question

###### Resultsi1. Using one sheet of standard (non-logarithmic) graph paper, plot the OD 600 on the vertical axis versus time (minutes) on the horizontal axis for each of the four tubes. Place all four curves on one graph Using the starting and ending OD values_ calculate the OD/min. for each tube: Note: The plots are not usually linear; thus exact slopes are difficult to determine. Construct table 2 to specify the OD/min for each sample:OD/min ((OD start OD finall/total minutes of assay)Calculate th

Resultsi 1. Using one sheet of standard (non-logarithmic) graph paper, plot the OD 600 on the vertical axis versus time (minutes) on the horizontal axis for each of the four tubes. Place all four curves on one graph Using the starting and ending OD values_ calculate the OD/min. for each tube: Note: The plots are not usually linear; thus exact slopes are difficult to determine. Construct table 2 to specify the OD/min for each sample: OD/min ((OD start OD finall/total minutes of assay) Calculate the specific activity for tubes 1-4. Average the specific activity for the mitochondrial suspension fraction (tubes 182) and for the negative control fraction (tubes & 4) Specific activity for tube 1, 2 = (OD/min)/(ug/ul of protein for mitochondrial suspension fraction) Specific activity for tube 3,4 = (OD/min)/( ug/ul of protein for negative control fraction) Stop and evaluate your results: The calculated specific activity is used to determine which fraction tubes &2 or 3 & 4) contains the highest mitochondrial concentration: Tube one and two theoretically should be higher based on the differential centrifugation scheme.