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# A gene that is normally expressed only in pancreatic cells is shown below_ The upstrcam region of this gene including the core promoter containing the TATA and CAAT...

## Question

###### A gene that is normally expressed only in pancreatic cells is shown below_ The upstrcam region of this gene including the core promoter containing the TATA and CAAT box (core promoter designated as D) and upstream regulatory region (A, B and C) are shown You fused the entire region (A to D) or part of it where cach of these regions were individually deleted to B-Galactosidase (LacZ) gene and then the DNA was introduced into pancreatic cells or into kidney cells_IAI B | Upstream region Core pro

A gene that is normally expressed only in pancreatic cells is shown below_ The upstrcam region of this gene including the core promoter containing the TATA and CAAT box (core promoter designated as D) and upstream regulatory region (A, B and C) are shown You fused the entire region (A to D) or part of it where cach of these regions were individually deleted to B-Galactosidase (LacZ) gene and then the DNA was introduced into pancreatic cells or into kidney cells_ IAI B | Upstream region Core promoter IacZ gene coding sequence The data in the following table are the results of 8-Galactosidase activity from this experiment. Region Deleted Cell Type Transformed Percentage of 8-Galactosidase Activity None Pancreatic 100 Pancreatic Pancreatic 100 Pancreatic 100 Pancrcatic None Kidney <l Kidney Kidney 100 Kidney <l Kidney If we assume that the upstream region has one silencer and one enhancer, answer the following questions: i) Where are the silencer and enhancer located? ii) Why don we detect the presence of the silencer in the pancreatic cells? iii) Why isn't this gene normally expressed in kidney cells?

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