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You are a researcher performing work on recombinant vaccines in a proteomics lab. You are focusing o RSV (Respiratory Syncytial Virus) and want to develop a subunit...

Question

You are a researcher performing work on recombinant vaccines in a proteomics lab. You are focusing o RSV (Respiratory Syncytial Virus) and want to develop a subunit vaccine for it: You know a vaccine for this disease will be boon for society; as it causes 160.000 infant mortalities annually. You believe the best vaccine candidate to be the surface glycoprotein G ofRSV. You have spent the past few months carefully extracting the genome of RSV and are nOW ready to begin your workTo extract the des

You are a researcher performing work on recombinant vaccines in a proteomics lab. You are focusing o RSV (Respiratory Syncytial Virus) and want to develop a subunit vaccine for it: You know a vaccine for this disease will be boon for society; as it causes 160.000 infant mortalities annually. You believe the best vaccine candidate to be the surface glycoprotein G ofRSV. You have spent the past few months carefully extracting the genome of RSV and are nOW ready to begin your work To extract the desired gene from the RSV genome, you decide PCR is the most appropriate method You refer to the GenBank database to find the genetic sequence for RSV glycoprotein G. What are the 5 ingredients that will be required for this PCR reaction point):



Answers

The laboratory you joined is studying the life cycle of an animal virus that uses a circular, double-stranded DNA as its genome. Your project is to define the location of the ori$\operatorname{gin}(\mathrm{s})$ of replication and to determine whether replication proceeds in one or both directions away from an origin (unidirectional or bidirectional replication). To accomplish your goal, you isolated replicating molecules, cleaved them with a restriction nuclease that cuts the viral genome at one site to produce a linear molecule from the circle, and examined the resulting molecules in the electron microscope. Some of the molecules you observed are illustrated schematically in Figure $Q 5-1 .$ (Note that it is impossible to distinguish the orientation of one DNA molecule from another in the electron microscope. You must present your conclusions to the rest of the lab tomorrow. How will you answer the two questions your advisor posed for you? Is there a single, unique origin of replication or several origins? Is replication unidirectional or bidirectional?

Okay. So I went there and did this and you should be able to see the same sequence up to 1/16 of the original size. I would go through with each thing that I found. But I feel like this is more of an independent learning activity. It does work if you go to the entrance search and you enter the values and you go through and look at your jeans and your probe and your unity. And I believe the purpose of this activity is for you to find that you can we have a really, really small fragment and still see the same sequence.

Here we're looking at different types of vaccine and we're going to match for use to some examples of the types we've been given are inactivated vaccines. Live attenuated vaccines. Toxoid recipient. Okay, let's look at the examples given. So A is a weakens influenza very on. It can only replicate in the lower temperatures of the nasal passages. So we don't get flu. We do get an active infection. So this is a weakened version, which means it's live attenuated. So inactivated has been killed, live Attenuated is still alive but less capable of causing disease. And then talks with Serbian are both only parts of the virus or bacterium or whatever. Let's look example B tetanus toxin molecules are harvested. So here we're looking at a smaller portion. It's not an entire pathogen, it's for toxin. And that means we're looking at toxoid. So toxoid is very similar to subunit but toxin specific or as subunit can be any anti camp saying we have influence the particles grown in chicken and mix, but a chemically treated to be non infectious. So here we have a virus has been inactivated, just going to be seen because we have the whole pathogen, but it has been treated services non infectious. And finally d we have the gene for a surface anti um and multiplied yeast is grown to produce a protein. So here we have a subunit, we have only a part of the pathogen. We have the surface antigen, which is going to produce an immune response without being infectious.

Hello everyone. Let's start this question. So in this question they gave fight test tubes. Okay, one to fight in these fighters TMZ does he call it? Let's find which we call it. Is infected with the battery of age that has phosphorus 32. Okay. The answer for this question is it is still one to stoop before and testify. Okay. I'll tell you what is the reason for this actually. The meaning of the bacterial fage contains fast for us and the protein contains a sulfur. When the bacteria of ages. In fact the cell they injected their D. N. A. Okay into the cell but the protein code stays on the surface of the cell. The protein court are shared of in the blender while the cells with the DNA pellet at the bottom of the tube. Okay. Cells with the DNA palette is at the bottom of the tube. Thus okay cells infected with sulfur 35 label bacterial fair will have radio activity associated with the protein got whereas those cells infected with phosphorus story to battery of age will have radio activity associated with the cells. So by using this thing for sulphur radio activities in court for prosperous radio activities himself. By using this we can find out 14 and fight is to ask the bacteria E coli which is infected with phosphorus story to label the bacterial face. Thank you everyone

So here we're continuing on with our work related to DNA, and we're gonna be taking about that restriction enzymes. So in the fast pothead of the types of plasmids that could be found with the T r a r with superscript on includes the original intact plasma with the eukaryotic gene fragment on like a tick plasmas with other plasma. It's instead of foreign DNA fragments, so moving on to the next part. So given the data that we have, so the lane wanted to have digested plasmids with a single eukaryotic gene fragment in different orientations. So after digestion, delaying one and two hard only to fragments with different molecular weights, whereas Lane three has a digestive plasmid with two eukaryotic gene fragments, so it yields three DNA fragments instead of two because it has to e c o r I cleavage sites


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