5

4: To assemble & genome You need to generate much more overall sequence than is in complete genome Why? Answer: 12. What impact would the length of your reads h...

Question

4: To assemble & genome You need to generate much more overall sequence than is in complete genome Why? Answer: 12. What impact would the length of your reads have on the number of sequences necessary to reconstruct an entire gcnome or transcriptome? Answer:

4: To assemble & genome You need to generate much more overall sequence than is in complete genome Why? Answer: 12. What impact would the length of your reads have on the number of sequences necessary to reconstruct an entire gcnome or transcriptome? Answer:



Answers

Based on Figure $14-2,$ why must the DNA fragments sequenced overlap in order to obtain a genome sequence?

So here were given a sequence and, um asked, We're told that the Cdn A sequences are lined with genomic sequence. How are CDs? A sequence is helpful and our seeding is more important for bacteria, or you carry its so CD and a, um defines the Exxon's of a particular gene. The sequences in between therefore represent the introns. Um, consequently, seeding days are more important for, um, UK erotic Gino annotation because bacterial genes lack introns.

Here asked would be faster to use CD and a clones in this method because they do not contain any intron sequences. So the answer is yes. This is because insurance sequences do not code for anything. And therefore, if we translated from emanate from M R NATO back to the DNA here, essentially, it's gonna get rid of that would make it faster for Part B here ASAP. What would happen if we encounter repetitive DNA sequences so repetitive sequences year can be eliminated through what we known us overlapping. So if we confined overlapping weaken, essentially eliminate these from the sequences in orderto help us create the library and the jeans.

This question is asking how alternative splicing increases the genomes coating capacity. So alternative splicing allows for one gene to encode multiple products, depending on the modular addition and removal of the relevant Exxon's.

So this question gives us, um, six different sequence reads, and so we're going to be using these six sequence reads to pray a sequence content of this part of the Gino. So you're just going to align the sequences to determine their overlap? Um, and once you overlap them according to where they would match up with each other, you create the, uh, kontic of, um um, then, since you do not know if the DNA sequence represents a temple Ishan for the Amorin A or is a complementary strength and there are two possible, um, transcripts. So you either turn all the cheese into use or, um, you will turn off the tees into a zone all the seas into G's and vice versa. So then that question be asks us to transit the sequence contact each of all possible reading frames. So the translation of the first possible transcripts starting at the first letter reads, uh, C l P Okay, translation starting at the second letter. So sissy. First, this is the second translation, starting at the third reads for the second part, services first and now for the second possible. This transcript, starting at the first letter. It will just read Stop for the second possible transcripts, starting at the second letter and then for the third. And now we have, ah, questions. See, that asks us to go to the blast page of the, um and C b i website and see if you can identify the gene. So using the nucleotide sequence of the con take and performing the blast, um, or the possible translation products and performing t blast in You will discover that the sequence in the translation product with above licit first match perfectly with the region of Exxon 19 of, um human. See if tr gene


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