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[29 1,2 ~sul 10" FlZxlo (5 pts) Prepare serial dilution of the gDNA, where the stock concentration is L.2ug/u. Each dilution has final volume of 100pl. StockFi...

Question

[29 1,2 ~sul 10" FlZxlo (5 pts) Prepare serial dilution of the gDNA, where the stock concentration is L.2ug/u. Each dilution has final volume of 100pl. StockFinalFinal Volume (pl)concentration of dilution (pg/ul)Concentration Volume of Volume of Dilution BDNA (pelu) BDNA (u) diluent (pl) Stock L2Ke Dilution Kko Dilution 2 3e" Dilution 3 Lo_ Dilution 4 2 Xe?Copy # of byl-2ISul

[29 1,2 ~sul 10" FlZxlo (5 pts) Prepare serial dilution of the gDNA, where the stock concentration is L.2ug/u. Each dilution has final volume of 100pl. Stock Final Final Volume (pl) concentration of dilution (pg/ul) Concentration Volume of Volume of Dilution BDNA (pelu) BDNA (u) diluent (pl) Stock L2Ke Dilution Kko Dilution 2 3e" Dilution 3 Lo_ Dilution 4 2 Xe? Copy # of byl-2ISul



Answers

(a) How many milliliters of a stock solution of $10.0 \mathrm{M}$ $\mathrm{HNO}_{3}$ would you have to use to prepare $0.450 \mathrm{~L}$ of $0.500 \mathrm{M} \mathrm{HNO}_{3} ?$ (b) If you dilute $25.0 \mathrm{~mL}$ of the stock solution to a final volume of $0.500 \mathrm{~L}$, what will be the concentration of the diluted solution?

So were provided with some data with concentration off stock industry solution as 14.8 mullah the volume off diluted solution as 1000 on the concentration off dilute solution as 0.250 So the formula off either delusion is m concert re concentrated equals m diluted multiplied with b diluted we're em concentrated is the polarity of concentrated solution We concentrated is volume of concentrated solution. M dying is equal to polarity off dilute solution and we dial formal volume off diluted solution. No substituting in the bad news. We have 14.8 multiplayer with the concentration into stew 0.250 multiplied bread 1000 mill. It gives us the volume of concentrated solution of 16.9 Emily so 16.9 Emily off stock solution is required to prepare 1000 mellow 0.250 m ammonia solution for option B. We are given data off stock solution off animals. The concentration off stock solution as 14.8 m on the volume off diluted solution is 0.500 leaders. From this, we can have Tzeitel 0.5 00 Neto multiplied with 1000 and then derided by one Neto, is equal to 500 ml no substituting the values in tow This given formula, so polarity off diluted solution will be 14.8 a. M multiplied with 10 animal divided by 500 million equals 0.296 and

And this problem. We're told that a 10 milliliters sample of a two points verified Mueller potassium nitrate solution is diluted to 250 milliliters. We would like to know what the polarity of the diluted solution is. To solve this problem. We can use this dilution relationship given here where we have the malaria and volume of our initial non diluted solution. And then we have that equal to theme polarity and volume off are diluted solution. So we know that the polarity of our initial solution is 2.5 Moeller and we have 10 milliliters of the solution we would like to solve for the polarity of our diluted solution and it has been diluted to 250 milliliters. When we solve for our mill aridity of the diluted solution, we find that it is equal 2.82 mil, where

Okay, so this problem is a simple AM on few on equal toe MTV to formula was started. The first bird you first. It's in the first part our, ah, we can consider the stock solution concentration as M one, which is the stock's risen Contreras six. Moller. And if we considered the falling on the stock solution required as few one, that's what we have to find out and came to hear is on the final or the resulting concentration which is point five Mueller and the resulting or the final four volume. Here is the volume of the resulting solution which is one hundred ten milliliters. So you have to find out the fuel on. So just put these values in this formal or were just rearrange it as em to veto over m one. That witch has put the values so himto. Here is your plan Fine Mourner Times The veto is hundreds, captain. Many later someone is six smaller from here are very one bell will be nine point one six militar. So this is the falling on the stocks solution we require Teo prepare hundred animal off point five Mueller Patrick is a solution now in the second part, we have to dilute the animal of the stock solution to a final volume of point two five three litre. If you find out, concentrate on the night. Your solution of the final solution again here or any one is a stock solution. Concentration one six Mueller V. One is what we're taking from the stock solution which is ten millimeter and our aim toe is the final concentration with you have to find out. And our veto, um is the final volume which is point two five zero liter. No, we have wanted Millie turn one of letters. We have to convert either one. So come back later, toe to fifty million liter. I have to find a veil off em too. So if we just really is the formal, I'm too will be em. One view on over V two. So everyone here is The stock's a different concentration. Okay, so it is not point six. If six smaller, it has six smaller. All right, so we have him six times. Then he shall follow him for the volume of the stock solution we're taking, which is ten million later. And the feet too is the final volume order or the volume the resulting solution which is two. Fifty militar. It will give us the final concentration, which is zero point two four Mueller. So this is the concentrate on the diluted solution if we dial ten militar off the stock solution to produce a solution of a bowling off point two five zero later or two fifty militar.

To answer this question and one B one equals two AM to be too. So that's 50 50 millimeter times 12 Moola equals to 0.1 more times veto, so v two equals two secs leaders.


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