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9_ What would you do if you wanted to visualize all of the DNA molecules within an actual mitochondrion? 10. What are two different methods you could use to confirm...

Question

9_ What would you do if you wanted to visualize all of the DNA molecules within an actual mitochondrion? 10. What are two different methods you could use to confirm that mtDNA is located within the matrix component of a mitochondrion? Describe using diagrams

9_ What would you do if you wanted to visualize all of the DNA molecules within an actual mitochondrion? 10. What are two different methods you could use to confirm that mtDNA is located within the matrix component of a mitochondrion? Describe using diagrams



Answers

Explain, and draw a diagram to show, how acetylation or phosphorylation could change the binding affinity between DNA and histones.

This question asked us to describe the main functions of the different components of the 80 Pecent days enzyme in the mitochondria. A structurally similar enzyme is responsible for the acidification of license OEMs and end disowns. Given what you know about the mechanism of 80 p synthesis, explain how this acidification might occur. Well, it's important to note that 80 p sympathies is going to be comprised of two components. The first component is F zero, and the second component is going to be F one. So they will in conjunction comprise the 80 p sin face machinery. Now F zero is going to be embedded in the inner might A con drill membrane and the F one complex will be connected to the F zero and it will contain the 80 p ace catalytic activity. So what we see is that one of the sub units that is going to be responsible for nucleotide binding will, um interact with 80 p and the free phosphate to release 80 p. Right, So basically, inside this 80 p ace, there will be a sub unit that allows for the union of identity and I phosphate with a free phosphate to form a teepee and 80 p is what we want. Now, uh, if we are to look at what happens in vessel acidification although, uh, instead of generating 80 p 80 p, ace will hide realize 80 p, which will then be coupled to Proton pumping against the greedy it so a kind of functions in reverse. So instead of using if we were to look at the my condom membrane right here, instead of having our 80 p sympathies right here, instead of allowing hydrogen ions to flow in powering the census of 80 p, we will do the opposite. Well, actually, hide allies 80 p will go in the other direction and will thus be able to pump hydrogen ions out in our vessel off the vast ical acidification. So that is how acidification in that A license so Miranda Soem will work.

Alright. In this question, we're asked to look at pro carry Ah, tick plasma membrane h ups. And this is verse, mitochondria and chloroplasts. So we're looking at our GP synthesis in proc chariots and organelles and you carry ox. So we know that they work similarly crazy, right? And we want to think about how may be thes organelles are related to pro Kerasiotes in some way. Andi, we have this evolutionary theory that organ Al's were once free living precarious. So our nice little bacteria ended up inside another cell. What crazy Now it just lives there inside of it. It makes its own energy from something free living and making its own energy to something bigger. It makes energy for the entire cell. So this is what we were called bio symbol Ayotte's. We just kept them around because they did good work for us, right? So now if you then think about thes 80 p synthesis is and what their amino add acid sequences would look like, say, we had them all lined up, there would be small to no changes. We imagine that there would be so similar our sequences would be so similar, and that's why we think that they came from free living bacteria

This question asked us to fill in the blank. The process of introducing dealing molecules into eukaryotic cells is called blank, so the answer here is going to be trance faction Transfer action is basically a process by which we add these DNA molecules into these eukaryotic cells with the hopes of these eukaryotic cells taking in the Deanna and incorporating it into their genome and eventually producing the M R name and protein associated with it. So after transfer action, we would want to see that there's Marnie, produced from the desired DNA sequence that has been inserted as well as protein and through trance faction, we should be able to alter the genome off the target you carry on excels. So the answer here is going to be transferred action.

Okay, So we're going to look at the role of March Contra carats X else as a quick review of earlier chapters. Notion of my country on produce 80 p. They carry out cellular respiration near the sites of cellular respiration. Um, which generates 80 p. Beefs as an energy source by the rest of it. So So you want to remember, he wants remember the Krebs cycle, and you want to remember be electron transport chain happening in the country.


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