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Pts) As a member of the phylum Actinobacteria M: tuberculosis groups with the gram-F positive bacteria on the 16S IRNA phylogenetic "tree of life_ However, it ...

Question

Pts) As a member of the phylum Actinobacteria M: tuberculosis groups with the gram-F positive bacteria on the 16S IRNA phylogenetic "tree of life_ However, it is colorless after a Gram stain . Explain how it is possible for M: tuberculosis to group with the gram-positive species on the phylogenetic tree yet also pot give positive Gram stain result:

pts) As a member of the phylum Actinobacteria M: tuberculosis groups with the gram-F positive bacteria on the 16S IRNA phylogenetic "tree of life_ However, it is colorless after a Gram stain . Explain how it is possible for M: tuberculosis to group with the gram-positive species on the phylogenetic tree yet also pot give positive Gram stain result:



Answers

Gram-negative bacteria a. appear blue to purple following Gram staining. b. appear pink to red following Gram staining. c. are all either bacilli or cocci. d. contain no peptidoglycan in their cell walls. e. are all photosynthetic.

Question. We have been after the bacteria. We does not take the gram stain. What do we call that type of bacteria. Soon before graham steaming we know that the sale of all or the cell of the bacterial cells have the health complex sell and we love which is composed of Australia that is like okay Alex followed by sand wall and then there in earlier of plasma membrane. Now these each layer of this bacterial cell envelope, it performed different functions but together act as a single protective unit. Now the cell envelope. Uh there are differences in the cell envelope of this bacteria which also uh differentiate them into two groups. That is grandpa, the Duke and gram negative. Now this the this differentiation of bacteria is done on the basis of the on the basis of how they respond to the staining procedures which was developed by Graham. That is gram staining. Now the bacteria which take off this grand stain they're referred to as the grand positive and we do not take the ground stain. They do not they're referred to us gram negative bacteria. So in the given question we have been all about the bacteria. They the bacteria has not taken the gram stain on the procedure. So what do we call that bacteria Now that bacterial we referred to as gram negative. So the correct option would the option B.

All right. So for this question we need to answer uh what do the following is pertaining to gram negative bacteria. Now, gram negative has been determined and classified as so due to a gram stain, which is very interesting because we can very easily determine if organism would fall in this category is just by doing a simple standing on a slide and we can either have to results an organism being gram positive or gram negative. And this can be classified uh further due to a structural composition of their cell wall. So we're talking about a gram negative is referring to the composition of the cell wall, which makes sense because it's where the dye uh the stains would stick to. Right? So what are the following is true about gram negative bacteria? Answer option A So after completing this gram stain to determine if it is gram negative and gram positive, it will be blue to purple if it is gram negative. And this is false because when we are uh this is going to the results for the gram positive gram negative bacteria will turn more of a violet or not violate a pink or red sort of color due to the alcohol would wash off the purple, the blue ish sort of die off of the cell wall where this wouldn't happen to the gram negative. So answer option B most abundant bacteria and this might be a little bit of a head scratcher. Because how do we know this? Alright. What information can we find to support this? Okay. Now, when we're looking at the different bacteria groups, the largest group by far the largest group of bacteria in terms of numbers, so the most abundant that's abundant means right? The most the highest amount most commonly found. So by far the largest number is going to be the proteome bacteria, pro. T. O. And I'll abbreviate this rodeo bacteria and these are classified many of them right as being a gram negative gram negative. So this is a very strong evidence that gram negative bacteria make up the majority of all bacteria. Because this is by far the largest group in terms of numbers, this would definitely be the most abundant, meaning this is true. So that is how we can easily understand this. We can further look at the other answer options and determine that they are also true. Which further solidify are inter option B being correct.

For this question. We have an instructor that's trying to get a mixed culture of bacteria and stain it accordingly. So here we have a grand positive cox C. As well as a gram negative rods. And we're also told that the grand positive cox C. Is going to be non model or in able to move. Whereas our gram negative rod is mortal meaning it has a flat gela and is able to move around and the fluid or media. So here we have the media or the tube that we are trying to incubate these bacteria in and we're going to inoculate it with our grand positive cox C. As well as our grand negative rods. So here the important differentiation between the two is the motility of each of these. So as we incubate the solution for 24 hours for 48 hours in order to give them proper time to grow the grand positive cox. Er not able to move throughout the solution because there is no agitation. This means they are pretty much fixed in place inside the solution. R. And media and are not going to be able to spread out and reproduce very well throughout the solution or abroad. However our gram negative rods, which are model are going to be able to move throughout the bra and able to reproduce all throughout the media that we are using. So the reason that our instructor when they are taking a culture of this and doing a gram stain, the reason that they are only seeing these gram negative rods is because these gram negative rods are able to out compete. Our grand positive cox C. And this is because they are better able to reproduce and divide because they are modal in this solution so they have much more space and much more nutrients to be able to utilize inside of this media. Whereas our grand positive cox C, which are fixed in place due to their non motility, are not going to be well able to utilize the space properly and the nutrients inside the solution. So here, for this question, the main reason they're seeing these gram negative rods is its ability to out compete the other bacterium, due to its increased ability to utilize space nutrients and reproduce inside the broth.

When we talk about drug resistance or antibiotic resistance, you could think about it in terms of evolution. So an anti biotic goes in and kills specific bacteria. Okay and all the susceptible bacteria killed off even when you use it when there's not an infection present. But any individual bacteria that are resistant to these antibiotics survived and therefore those individual resistant criteria of greater fitness. And they multiply and they reproduce, which reduces the future effectiveness of the antibiotic treatment. So it three antibodies are used. Often it creates environment in which the resistant bacteria have greater fitness and multiply and are more prevalent.


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