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# Part li Cytotoxicity Tertest the effect of Drug A on breast cancer cells, pertformed cytotoxicity assay (WST1) was Drug _ is prepared in DMSO as concentrations of s...

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###### Part li Cytotoxicity Tertest the effect of Drug A on breast cancer cells, pertformed cytotoxicity assay (WST1) was Drug _ is prepared in DMSO as concentrations of solvent, and serial dilution is done to obtain different Dryne A (500, 300 , 200, 100 , 75_ added to each well_ and 25 pM) with stock bottle 5OuL of drug to be you started with has The absorbance of the different concentration of 1OOOpM results obtained_ wells was then read on the ELIZA machine and the followingConcentration SOOpM 300

Part li Cytotoxicity Tertest the effect of Drug A on breast cancer cells, pertformed cytotoxicity assay (WST1) was Drug _ is prepared in DMSO as concentrations of solvent, and serial dilution is done to obtain different Dryne A (500, 300 , 200, 100 , 75_ added to each well_ and 25 pM) with stock bottle 5OuL of drug to be you started with has The absorbance of the different concentration of 1OOOpM results obtained_ wells was then read on the ELIZA machine and the following Concentration SOOpM 300 pM 200 HM 100 pM 75 HM of Drug 50 pM (pM) 25 pM Absorbance 3.065847 2.908687 2.85327 2.686384 .894488 (OD) 1.310001 0.003333 Describe in details the protocol used to perform this experiment starting from plating of cells (without numbers) then through serial dilution (with calculations) up until the reading: What could be said about Drug _ A? Why? (Illustrate if possible) control, another plate with only DMSO (without Drug was prepared: What would be the shape of the curve (DMSO concentration vs: Absorbance) if DMSO has no effect on the breast cancer cells? What if it had killing effect? What if it had proliferative effect?

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