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Which of the following is least likely to be used to identify gene in an unannotated sequence of DNA? Polyadenylation cleavage site Cis regulatory element TATA box ...

Question

Which of the following is least likely to be used to identify gene in an unannotated sequence of DNA? Polyadenylation cleavage site Cis regulatory element TATA box 5' splice site Hormone response element Open reading frame Restriction enzyme site

Which of the following is least likely to be used to identify gene in an unannotated sequence of DNA? Polyadenylation cleavage site Cis regulatory element TATA box 5' splice site Hormone response element Open reading frame Restriction enzyme site



Answers

Which of the following facilitates joining a piece of human DNA with bacterial plasmid DNA? a. DNA polymerase c. RNA polymerase b. DNA ligase d. restriction enzymes

Which of these enzymes can cut DNA specific regions is it's a like is the healer case. CPL. Emery's body restriction enzymes. So let's look at legs, legs, glues, DNA together. So the opposite of what we want. Hello, case it can separate double stranded DNA into the two strands, which is useful. Brought both transcription and, uh, replication. But it does not cut the DNA, and it's also not specific. Preliminaries replicates DNA, which leaves our answer restriction enzymes. They recognize a specific sequence of D. N A and B cuts for Double Strand at that point.

This problem asked us to distinguish between the cloning methods used for eukaryotic genes like ours and pro periodic jeans like the genes from a bacteria both you carry its and pro corrodes have D. N. A. And they use that DNA via transcription to make MoreNA. That can then be translated into proteins. So let me quickly label my DNA and my camera here. Once the Mrna transcript is created though the processes diverge a little bit in probiotic cells, genes can be directly translated off that Mrna transcript. In fact it can happen literally while the transcript is getting created in eukaryotic cells, there's going to be a step of processing to help the Mrna get out of the nucleus. And also just to do some some editing along the way. The most relevant editing for us right now will be knowing that there are segments of the DNA that are non coding segments called introns that are going to be spliced or cut out. So we're going to take our Exxon's or the parts of DNA that do code splice them together and attach them, add the cap and tail so that the camera is able to get out of the nucleus. And this entire processing process happens to every single piece of Mrna that gets expressed in the eukaryotic cell. And that's all well and good. But it means that when we give this March and when we give a gene to a bacteria it won't know what to do with these introns. So if we're going to create a piece of DNA that we can use in a plasmid cloning situation, we're going to need to use a special tool called reverse transcriptase that can create D. N. A. From an MRNA. A processed MRNA transcript. And this is called C. D. N. A. Or complementary DNA. So that's basically going to be a copy of the original DNA strand. but with the parts that were spliced out of the MRNA removed, so it's only the useful parts of the gene. So our answer here for what do we need for eukaryotic cloning but not pro carry on. Cloning, is c reverse transcriptase.

This problem gives us a sequence and it's probably about 50 nucleotides long, and you can see that I obviously shortened it. But it wants us to find the transcription start, start site, at least, so most likely one in this secrets. So I was going to point out a couple key consensus sequences that make this a lot easier. Um, so I didn't write every school nuclear tied down, but I kind of took three groups of nucleotides in this whole sequence. That's gonna be really key for us to solve this problem. So the 1st 1 I looked for was around this one on. This is the exact sequence of the prim Bo sequence where the prove no secrets, which happens that negative 10 in procuring is common there. So I found this. And then the second thing I looked for waas upstream from that, this sequence right here is characteristic of the negative 35 element in per carry. Its it's exactly snatches up exactly the consensus secret. So this starts at negative 35 and we have the privilege of sequence that negative 10. The next thing I did was I just counted out until I got to, Uh, first, I got to zero, which is also plus one, which is the transcription start sites. So that turned out to be this G 10 nucleotides upstream from the pregnancy Quince. So this is a plus one, and this is our transcription start sites right here.

In here us that the DNA is derived from a middle of a city and a loan of a 1,000,000 protein. So it asked us if we can determine that amino acid sequence of this portion of protein. So essentially it is not going to be possible due to the fact that from the information that we're given, we cannot determine the amino acid sequence here because of the three reading frames, all of which do not contain a start code on here. And that's the reason why we cannot tell anything about this particular sequence. Um, and that's going to be for part B. For part eight. Ask what's the sequence of DNA? And that's used in the sequencing reaction shown here. So we have four legs that show the products of sequencing reactions that contained the DDG D D A and DDT, which are video nucleotides, which are used in singer sequencing on Essentially in orderto determined the sequence itself. We look at what DeOssie died, the oxy I'm nucleotide that to be used for example, G g a. T versi here, and essentially whatever is gonna end up at the last year is going to be the sequence. So we go from bottom top in this particular case,


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