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Which of the following changes in _ protein CANNOT be detected using mass spectrometry?Cleavage with an endoproteaseGlycosylation_Cleavage with an exoprotease:Phosp...

Question

Which of the following changes in _ protein CANNOT be detected using mass spectrometry?Cleavage with an endoproteaseGlycosylation_Cleavage with an exoprotease:Phosphorylation.All of the above CAN be detected with mass spectrometry

Which of the following changes in _ protein CANNOT be detected using mass spectrometry? Cleavage with an endoprotease Glycosylation_ Cleavage with an exoprotease: Phosphorylation. All of the above CAN be detected with mass spectrometry



Answers

Post-translational modifications of proteins can affect which of the following?
a. mRNA splicing
b. 5’capping
c. 3’polyadenylation
d. chemical modifications

Here we are talking about different factors of post transcription. Elrod regulation or control. So as the word indicates, this is going to be anything that occurs after the transcription of the M. R. N. A. So you can imagine that you have your DNA strand with the gene of interest. And your problem arises will cause the creation of what we call the pre M. RNA strand. That pre M. R. N. A is going to undergo processing inside the nucleus in order to be converted into the finalized M. RNA strand. And that of course can go through the process of translation and the ribosomes in order to create your protein. So here when we talk about this post transcription ALS regulation, we are talking about this processing strep step to change the pre M. R. N. A. To the MRNA strand. So that's going to include a few modifications. It's going to include the addition of a cap to one end of the RNA and the addition of a poly a tail. And those are going to allow it to survive better in the cytoplasm where you have digestive enzymes that we would call our and aces. So these poly A tail and caps would prevent the R. And aces from digesting the important end of the MRNA strand and the cap allows it to be recognized in the river zone as well. In addition to those processing is also going to include the process of alternative splicing. An alternative splicing is where we are going to cut out certain sections of the MRNA. So you can imagine that your M. R. N. A. Includes several different gene groups. So alternative splicing can split out certain genes within their and it's going to litigate the two ends together. And from there you'll get a different product which will produce a different protein based on what's needed in the cell. So here the post transcription regulation is going to include the processes of alternative splicing and the addition of the cap and poly a tail to the M. R. N. A. For the other answer choices. You have the ubiquity ation and you have the protea elliptic digestion. And these are processes that are going to modify the protein strand with the addition of a ubiquitous own group, which will allow it to be recognized for destruction in the produce ozone. So those are more regulations of the protein rather than of the MRNA strand. So you can eliminate those answer choices because they are not post transcription ALs because they're working on the protein itself. As for foss for elation, this is the addition of a phosphate group to a protein and that's typically going to activate or in some cases it can deactivate the protein. But again, that's working on the protein molecule rather than the M. RNA itself. So here are the only post transcription process is going to be this control of alternative splicing. So here you should take answer choice A.

I've Eiseley solved problem 81 45. So the problems is using oxidative and privilege helping really on distinguish between this too urging come bones. So for the oxidative cribbage the region that we need his petition permanganate in prison self acid. So when we drink the oxidative cleavage, we're going to break the problem carbon double bond in these two positions and want we're going to get these we're going to get it to carbon Looks like I guess it end for problems problems that upset a guess it Let's draw the five obsolete offensives. So these are going to die of obsolete gas it and we're going to get this one. So what we're going to use sucks only guessing and all presented rancid from the first trump on Now, on the 2nd 1 This I want government double bond is going to break in, and we're going to get Do only go. So CIMB said, we're going to have 1233 car bombs. So one carbon second woman time to buy one and we're going to get two months off this into the name off Thes Cassidy's Melanie tested my lonely guess it therefore using this oxidative privilege, we see that we're going to get to different, completely different products from the given compounds. And using this reaction, we can differentiate between these two compounds from there brought it.

The question here won't ask us to classify each of the proteins according to its function. So eight here is going to be insulin, and we know that that is a hormone that is produced to basically break down, Um, glucose be here. We know our antibodies and antibodies were produced by the white blood cells in order to disable pathogens or foreign particles. See, here is casing, which is a hormone that is produced by the milk and D here is like paces, which basically is an enzyme that helps break down or high July's various lipids.

Now this is one of those questions were just need to memorize the steps and specific details of the classes of the different signaling pathways. Now the activation of Artie case or receptor tires in kindnesses protein kind. These molecules and the regulation of transcription all require fast for elation. So all those are incorrect. The correct answer is C g coupled protein receptors. Now, this may seem incorrect at first, but if you look at this diagram closely, you can see that GDP doesn't really become fuss for related to become GTP. It merely it leaves the receptor and GTP takes its place, and this doesn't count as phosphor relation. Now this is a detail that's very easy to miss when studying, so memorizing specific things like this is very important.


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