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Below are examples of Restriction Enzymes with their sequence recognition and cut sites Which enzyme (s) represent(s sticky end cut?Not I:5 . GcGGCCGc 43 3" C...

Question

Below are examples of Restriction Enzymes with their sequence recognition and cut sites Which enzyme (s) represent(s sticky end cut?Not I:5 . GcGGCCGc 43 3" CGCCGGCG ..5'Haelll: 5 . GGcc 3" 3" CCGG 5"EcoRV; 5 . GATATC ..3' 3 CtAJ AG 45"Hindiwl AAGCTT .3' TTCGAA 5"

Below are examples of Restriction Enzymes with their sequence recognition and cut sites Which enzyme (s) represent(s sticky end cut? Not I: 5 . GcGGCCGc 43 3" CGCCGGCG ..5' Haelll: 5 . GGcc 3" 3" CCGG 5" EcoRV; 5 . GATATC ..3' 3 CtAJ AG 45" Hindiwl AAGCTT .3' TTCGAA 5"



Answers

Using the data in Table $3-2,$ identify restriction enzymes that (a) produce blunt ends; (b) recognize and cleave the same sequence (called isoschizomers); (c) produce identical sticky ends.

Let us complete the following sentence. In cloning, restriction enzymes serve the function of what Now, let us make a list of possibilities that can make the statement true and decide of all of them are accurate, only some of them. So in cloning restriction enzyme served the function of are used to cut DNA into pieces for gene cloning are produced by bacteria cells to prevent viral infection and or produce sticky ends and DNA fragments. After looking at all of our possibilities, we see that all of the above are true of restriction enzymes.

So for a stirring this question reality for the restriction enzyme that can produce plan and 40 meaning off blank and innings that when the restriction enzyme cast it has not produced over hen eggs for DP and a threat month. So this is called blanking and disease cost ki n? Because, oh, the's park, it is overhanging. Therefore, by looking at the enzyme Karsay, the answer is B.

So the first part of this question is asking about um the bacteria's produced restrictions uh design and so bacterias yeah yeah do produce restrictions also known as R. E. But they don't cut their own Vienna with that. So this is how so this is a plas make our restriction in Zion. We'll make cut from here to here. So this will government credit something like this. And then you can insert a new piece of DNA here. Uh huh. Mhm. So Part two of the question is asking features are restriction side so it is also known as restriction yeah restriction did have combination side. Uh huh. Okay. Okay. And bacteria is produced to contempt the court for in the union. Okay. Mhm. What? Yeah thanks. Also please let me think And according to the 3rd and the vehicle it is that it is located in DNA molecule. Yeah. Yeah generally all enjoyed. Okay. Yeah sequences. Mhm. Yeah Part three is telling us about blunt end. Oh oh yeah overhanging. Yeah yeah yeah. Okay. three crime and and also bringing at five prime and oh for one thing mm mm. Yeah.


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