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The graph hcre dcpicts the oreninria of boc operon in E coll: Pkay Insuat @e folloning qucslions50. Which 0f the following eenk trods & cnzymc calied Pgalarlosi...

Question

The graph hcre dcpicts the oreninria of boc operon in E coll: Pkay Insuat @e folloning qucslions50. Which 0f the following eenk trods & cnzymc calied Pgalarlosidesc 910 #LrZ dk}SE#hch oftc Iollowing cnoots urrptrar} Prokzin Iox the Ixr opkron? 2) 0 #Lrz dLer 82 Which o the folloning b t taddrzs o RNA Peh netase 9)0 a1z dL4!Whrnyou JJOV E celicclk m tut containing elucos bu no Extuk#ta4n Tollowing gan is cxpressedz c) Lacy Kla4nrtr 6) LxA dLkZsawnjo" gow E cali cclb mntket comining Iect

The graph hcre dcpicts the oreninria of boc operon in E coll: Pkay Insuat @e folloning qucslions 50. Which 0f the following eenk trods & cnzymc calied Pgalarlosidesc 910 #LrZ dk} SE#hch oftc Iollowing cnoots urrptrar} Prokzin Iox the Ixr opkron? 2) 0 #Lrz dLer 82 Which o the folloning b t taddrzs o RNA Peh netase 9)0 a1z dL4! Whrnyou JJOV E celicclk m tut containing elucos bu no Extuk#ta4n Tollowing gan is cxpressedz c) Lacy Kla4nrtr 6) LxA dLkZ sawnjo" gow E cali cclb mntket comining Iectoxs bu E0 Eluconc -hhdts following Ertk & eaprcesed? HLY 9aet De€ LZA DL Ss,Khayou Ept E celi ccik m##n conlainin both EeoreIdeesr ehch #n followving Ecnr #ePG fil JNNenae b)aLacA dLaZ



Answers

As a genetics historian, you are repeating some of the classic experiments conducted by Jacob and Monod with the lactose operon in $E .$ coli. You use an $\mathrm{F}^{\prime}$ plasmid to construct several $E$ coli strains that are partially diploid for the lac operon. You construct strains with the following genotypes: $(1) I^{+} O^{c} Z^{+} Y^{-} / I^{+} O^{+} Z^{-} Y^{+}$ $(2) \quad I^{+} O^{c} Z^{-} Y^{+} / I^{+} O^{+} Z^{+} Y^{-}$ (3) $I^{-} O^{+} Z^{+} Y^{+} / I^{+} O^{+} Z^{-} Y^{-}$ (4) $I^{s} O^{+} Z^{-} Y^{-} / I^{+} O^{+} Z^{+} Y^{+},$ and $(5) I^{+} O^{c} Z^{+} Y^{+} / I^{s} O^{+} Z^{-} Y^{+}$ (a) Which of these strains will produce functional $\beta$ -galactosidase in both the presence and absence of lactose? (b) Which of these strains will exhibit constitutive synthesis of functional $\beta$ -galactoside permease? Which of these strains will express both gene $Z$ and gene $Y$ constitutively and will produce functional products (\beta-galactosidase and $\beta$ -galactoside permease) of both genes? (d) Which of these strains will show cis dominance of lac operon regulatory elements? (e) Which of these strains will exhibit trans dominance of lac operon regulatory elements?

Certain talk about the lack Opteron, which is how we control if the enzymes that break down lack toes are made or not. Now the reason that we want to control this he's because you don't want to be making these enzymes that break down lack toes. There's no lack to its present. So normally it exists like this with this giant repressor sitting on the operator. And what a repressor does is it is going to lock transcription, which I'm an abbreviated as a T exits blocking transcription over Brockington transcription. We can't obviously do translation to make the proteins that would then help to break down the laptops. So this is what is happening when we have no lack chose present. Now, if we add some lack, chose to the system over gonna see happen is the repressive is going to leave. So without the repressor, it will just erase it away. What we're going to get is we're going to get transcription of the genes, so without repress, er equals transcription of collapses, enzyme genes. So now I want to think a couple of hypothetical situations to determine what would happen. So situation a is going to be. We have a bad, um, repressor, meaning it doesn't work, so does not bind. So if we have a repressor but can't find her, operator, what we're going to have happen is we're always going to be doing transcriptions. So always t X transcription of black toes and Zain. Jeez. So we're always going to be making the locked with enzyme genes, even if there's no watches. President. So another situation want us to think about is what happens if our operators So we have broken operator, meaning that's their oppressor Can't bind to it. Well, what's gonna happen is going to be extremely similar to what happened in situation. Hey, when we have the bad repressive, we're always gonna be transforming because we can't inhibit. If the repressor cannot find because the offer on his faulty we're always gonna be transcribing those genes. Now, the last thing I want to add is the mutation of a regulatory meaning that the repressor will not find the out So regulator G. And when trying that a regulator to you are talking about a gene that's up here, that's up street. And what this does is this gene oftentimes recruits the repressor to combine. But if this gene is faulty, we're not built to recruit that repressor. So once again, we're always going to be transcribing, even if there is no lack chose for us. So essentially the moral of the story that if you disrupt any part of this cycle, that's off regulation. So if you disrupt the repressor, the repressors ability to behind or those regulatory genes that recruit the repressor, what you're going to have happen is you're always going to be transcribing the lack jeans, even if there's no lack to its present.

Here, we have a multipart question. So for part A, we have the prompt that reads a mutation that prevents the binding of the drivers up to the five time end of the M R and a five time untranslated region. What effect will this half? Well, the answer is that no gene expression will occur for part B. We're looking at a mutation that changed the TRP code ons in region one of the M R five prime untranslated region into co dance for Allan E. So what occurs in this situation? Well, if those Allen and Co. Downs were replaced by trip to fan coat on, then under conditions of Low Allen in the stalling of the Riva Zone will not occur. So the attenuated will form stopping transcription derives, almost stalled when all in the alunan is low. So transcription structural genes will occur only when Allan E is look right. Um, so that would be our answer right here. That the fact that in attenuate reforms that starts transcription all right? No, the RAV ism will stall when all Alan in his left. So the transcription of the structural genes so transcription of those structural genes in the opera will only occur when Alan in is low. For part, C mutation creates a stop code on early in region one of the M R and a five prime untranslated region. So in this case, transcription will not occur because the regions three and four are now free to form the attenuated. So Regions three and four can form the attenuated purport D deletions in region two of the m R five prime untranslated region will cause an attenuate er to form and transcription will not look her part e we have that our delish ins in the region three of the m r five prime untranslated region will cause that TRP operate on to be unable to form the attenuate er in region three due to the fact that Region three contains that delicious So attenuation does not occur due to the fact that we do not have, um we have that delish in in region three. Since we do not have a good copy of Region three, which results in continuous transcription of the TR key structural cheats moving on to f, we are giving the problem deletions in region four of the m r five prime untranslated region. Well, these will prevent the formation of the attenuate er by the five prime untranslated region Marnie causing transcription to occur. And finally, with G, there is a deletion of the string of Adami nucleotides that followed region for in the fire prime you tr well, for the attenuate er hairpin to function as a terminator. That's obvious presence of a string of your soul nucleotides following following region for in in the m r and a five prime you tr. So the delicious of this string of adenine nucleotides in DNA will result in no string of your so nuclear times following region for so no termination occurs because we do not form that attenuate er and thus transcription proceeds.

You should try new. I went the first room on the table. These were so you find out for me that if two genes were apart, you're not praying their frequencies. So please, Forman's that's using the 1st 1 This rich would be your EUR 4.3 you. One year. No playing for person, which will give you 21 7 person. Well, what? We're getting pregnant. Seven? Yeah, ground 0% Which is greater. So they would show the product will be difficult here. The to transform its 4.3 times playing for she's gonna be your point one's point on 17 And you know what? Be a factor. Tune greater so that would you 0.17 person and that is here. Percentage of cool transformed her pert B is when two genes We're on separate fruit pieces of beginning then, uh, rain. Uh, cool transformation. It's no, which is concluded in

The concentration off. So any time that is the effect of the mutation on enzyme activity. The maximum rate achieve decreases when there is increasing of struck concentration. So V max is decreased and chemise increased Here, the wild type of Imax's 13 Onda more minute universe and universe the wild type came 2 45. both here in this both TV Max on came on the crest Wild type man. Why did type be Max is what they want and when they're turned worse on getting voice on the my father while the type Kim is 104. No helm. You turned to be Max. Where is Yeah, B match is 8.3 and yeah, minute inverse. And she in verse mutant k m a 69. Um, I think he was really affect off by the new CEO. Eight of the wild type mutant. And time is shown in a given figure expression. While that is much more sensitive to melon etc. Okay, The part the most prominent effect off the mutation on the properties of enzyme are as follows the A mutant. This list about 65% off the activity on the Nativity off the world with respect profoundly dine. It is waste 50% with respect of parameter c away also Then you off million Elsa in inhibitory already percent on the world's type but does not have on effect on them You turn in time a part of the functional Rohloff glutamate three in Canada tiene aside. Transfer is I seem to play a more significant role in relation My Ma Elena CEO a then in cattle Isis.


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