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Exercise C: Calculating transformation efficiencyTable 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentr...

Question

Exercise C: Calculating transformation efficiencyTable 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentration Total DNA Transformation DNA Diluted? of DNA of DNA (nglul) used in transformants efficiency added? used transformation (# of colonies) Y or N (M) (ng)t LBA nla nla; LAWN nlaLBA1010 nglulnla, LAWNnlaLBAampnlaLBAlamp1X (not diluted) 0.5X (diluted by_121020 nglulLBAlamp1010 nglul#To calculate "total DNA used in transformation

Exercise C: Calculating transformation efficiency Table 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentration Total DNA Transformation DNA Diluted? of DNA of DNA (nglul) used in transformants efficiency added? used transformation (# of colonies) Y or N (M) (ng)t LBA nla nla; LAWN nla LBA 10 10 nglul nla, LAWN nla LBAamp nla LBAlamp 1X (not diluted) 0.5X (diluted by_12 10 20 nglul LBAlamp 10 10 nglul #To calculate "total DNA used in transformation_ multiple volume of DNA used and concentration of DNA together (the volume units should cancel out):



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Components of $E .$ coli $E .$ coli cells are rod-shaped, about $2 \mu \mathrm{m}$ long and $0.8 \mu \mathrm{m}$ in diameter. The volume of a cylinder is $\pi r^{2} h$, where $h$ is the height of the cylinder. (a) If the average density of $E .$ coli (mostly water) is $1.1 \times 10^{3} \mathrm{g} / \mathrm{L},$ what is the mass of a single cell? (b) $E$. coli has a protective cell envelope $10 \mathrm{nm}$ thick. What percentage of the total volume of the bacterium does the cell envelope occupy? (c) $E$ coli is capable of growing and multiplying rapidly because it contains some 15,000 spherical ribosomes (diameter 18 $\mathrm{nm}$ ), which carry out protein synthesis. What percentage of the cell volume do the ribosomes occupy?

Hello, everyone. This is Ricky Hand today. We're working on problems. 59 check. And what would we use? Klaus? Mid transformation. We would use it to make copies of DNA like, for example, if we find a cool gene that we wanted to replicate and then uncertain to other genomes. We could do that by using club Mistress Klaus mid transformation.

On the image off the given caution. It is absurd. Able that plate that only have ampicillin resistant bacteria includes played number four, and the correct answer is option C.

You should try new. I went the first room on the table. These were so you find out for me that if two genes were apart, you're not praying their frequencies. So please, Forman's that's using the 1st 1 This rich would be your EUR 4.3 you. One year. No playing for person, which will give you 21 7 person. Well, what? We're getting pregnant. Seven? Yeah, ground 0% Which is greater. So they would show the product will be difficult here. The to transform its 4.3 times playing for she's gonna be your point one's point on 17 And you know what? Be a factor. Tune greater so that would you 0.17 person and that is here. Percentage of cool transformed her pert B is when two genes We're on separate fruit pieces of beginning then, uh, rain. Uh, cool transformation. It's no, which is concluded in

Hang on his limbs stroke from country. In this problem, we need to calculate the length off equal idea in a molecule. It is given that the one of the rate of equal idea is 3.1 multiplied by identity. Carmine, Grandpa, Mom, Everyone weight off Nick. That I bear is given 6 60 grand per month on the length office included Berries 0.34 nanometers. There was a number off the current. There is the A molecular weight off equal idea in a molecule divided by the average one were weight off nuclear trick bear, which gives us and therefore the lint off Dana Monica is Yeah, mhm the twenties 4696970 multiplied by 0.34 nanometer and we can convert it to millimeter by multiplying can Universe six, which gives us 1.6 millimeter. Therefore, the total length off equal idea in a molecule is 1.6 millimeters and this is 800 times longer than the cellular DNA. There from the beginning is highly gold. The next problem and need to calculate the maximum number of protein. Let's do that, given the length of the average Looking is 400. Um, I know said and each, um in the city's guarded by three letter code and therefore we can say each time in the S and is coated by a three letter bottom. So each group, it consists off three races, therefore the number of base it can do the length of the average protein, which is 400 multiplied by the number of basis three basis, which leaves US 1200. Therefore, a literal number off basis. We have calculated which is total number off basis. We calculate it. Therefore, the number of proteins coded by this econ idea and there will be this is a can turn the total number of races divided by the number of faces in this problem, which gives us 3914 proteins never


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