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# Exercise C: Calculating transformation efficiencyTable 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentr...

## Question

###### Exercise C: Calculating transformation efficiencyTable 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentration Total DNA Transformation DNA Diluted? of DNA of DNA (nglul) used in transformants efficiency added? used transformation (# of colonies) Y or N (M) (ng)t LBA nla nla; LAWN nlaLBA1010 nglulnla, LAWNnlaLBAampnlaLBAlamp1X (not diluted) 0.5X (diluted by_121020 nglulLBAlamp1010 nglul#To calculate "total DNA used in transformation

Exercise C: Calculating transformation efficiency Table 2 Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA Volume Concentration Total DNA Transformation DNA Diluted? of DNA of DNA (nglul) used in transformants efficiency added? used transformation (# of colonies) Y or N (M) (ng)t LBA nla nla; LAWN nla LBA 10 10 nglul nla, LAWN nla LBAamp nla LBAlamp 1X (not diluted) 0.5X (diluted by_12 10 20 nglul LBAlamp 10 10 nglul #To calculate "total DNA used in transformation_ multiple volume of DNA used and concentration of DNA together (the volume units should cancel out):  #### Similar Solved Questions

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