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You are studying a strain of bacteria that has boththe lac operon andthe trp operon.You place this strain of bacteria in 3 petri dishes, eachcontaining a different ...

Question

You are studying a strain of bacteria that has boththe lac operon andthe trp operon.You place this strain of bacteria in 3 petri dishes, eachcontaining a different medium.Petri dish 1 contains both tryptophan and lactose.Petri dish 2 contains neither tryptophan nor lactose.Petri dish 3 contains tryptophan, but does not containlactose. Name the operons that will be expressed in each petridish.

You are studying a strain of bacteria that has both the lac operon and the trp operon. You place this strain of bacteria in 3 petri dishes, each containing a different medium. Petri dish 1 contains both tryptophan and lactose. Petri dish 2 contains neither tryptophan nor lactose. Petri dish 3 contains tryptophan, but does not contain lactose. Name the operons that will be expressed in each petri dish.



Answers

Bacterial cells can take up the amino acid tryptophan (Trp) from their surroundings, or if there is an insufficient external supply they can synthesize tryptophan from other small molecules. The Trp repressor is a transcription regulator that shuts off the transcription of genes that code for the enzymes required for the synthesis of tryptophan (see Figure $8-7$ ). A. What would happen to the regulation of the tryptophan operon in cells that express a mutant form of the tryptophan repressor that (1) cannot bind to DNA, (2) cannot bind tryptophan, or (3) binds to DNA even in the absence of tryptophan? B. What would happen in scenarios $(1),(2),$ and $(3)$ if the cells, in addition, produced normal tryptophan repressor protein from a second, normal gene?

For this question, We are taking a look at the lack opera in jeans. So this is a sequence inside the DNA that is responsible for producing the lack jay's enzyme responsible for digesting lactose inside the bacterial cell. So this is going to consist of multiple parts. You have the operator and you have the black genes such as lack zine, black, white and like a And of course at the beginning of this, you will have the promoter where the preliminaries is going to initially attach. So here, in a normal process, the operator is responsible for turning the sequence on or off, meaning if the operator is bound and repressed, these black jeans will not be transcribed, whereas if the operator is unbound and free, it will allow lac case to be made through the transcription and translation of these different genes. So here we are being asked to consider a certain mutation in one of these genes, such as say, in addition mutation or through a deletion or some other sort of mutation. These mutations do not have an effect on how the gene and DNA sequence is red. So regardless of how these jeans look or their nucleotide sequences, as long as this lack operator is on the preliminaries is going to transcribe these three genes. What the mutation does though is the MRNA that is produced through this process is going to contain that complementary mutation as well. So here the mutation inside one of these genes is going to be reciprocated to the MRNA strand itself. And of course when the MRNA strand is translated, the proteins being made from the MRNA strand are going to also contain that mutation as long as a different amino acid is coded. So here the results of a mutated gene inside of this lack opera is going to result in a mutation in the protein strand itself. However, transcription and translation are still going to occur at the same pace, with no problems at all, because the problem is only in the coding and the sequencing itself, rather than in the function of this process. So here the best answer choice is going to be d that the only result of this mutation is going to be in the function of the protein itself, rather than in the actual translation and transcription process, which should occur normally.

This question has a picture of a plate with white and blue colonies representing a medium with ex scout. We'll draw the white colonies here at Black so we can see them. The blue colonies will have functioning. Medical Glock decide is because we know from the question, got beautiful inside days. Presentation can result in blue fragments. So I'm going to use a red color to circle blue colonies. So that's saying we have one right here, right here. Yeah, right here. The white colonies are the Black Sea and black White means because they won't have been a great societies Politicals in green. I think I was all three of them right here, here, here and here. So let's suppose we looked at LAX e and Y genes. I found a wild type Peter type. This might suggest that the promoter was actually meted it and other genes so that our people a race couldn't buying two d n a. And we wouldn't get transcription

Questions. Sex. We're talking about a Peron's come comparing processes Seenu procure. It's in new periods, so I'm pretty roots. We have one single operation where we have many proteins around this happen because it services the one promoter nothin creeds the production of Emory name. And so, since we have this just this one, um, stop around here forcing things. We see that a lot of proteins are created from this one more in a that all work in similar pathways and similar mechanisms therefore creating whatever process that needs to be done by this, um, making of this protein and in precarious what's this is happening in bacteria. So everything is happening Localized this in place the second that memory gnaeus um created. Then you see that being transcribed into protein it was a very I'm quick moving process. But then we look atyou. Carry outs, difference. Um, what a difference in the carats. First, they have a five prime. It ended in a poly a tail, which the procurer doesn't. They don't have ah Opteron. Instead, they're in tronic sequences of the Internet, which is drawn here. Um, is removed in order to make mature our name So is peace. Um is cut out, and instead we have these two sides completely joined together. So, um, this mature or in a is actually made in the nucleus and, uh, new periods. The process of forming a marinade into protein because of the right was almost which are in the cytoplasm. So not only is the same displace that needs to be in its final form before it leaves the nucleus, enters into the cytoplasm and then continued into protein, therefore, carry oats have more finished of, ah, protein products, and it has a couple more stops that could be skips through and could be causing more errors and procure.

This question asks. Lack those digestion in E. Coli begins with this hydrologist by the enzyme beta angle Acto cities. The gene encoding beta collective cities. Black Sea is part of a coordinated Lee regulated Opteron containing other genes required for lack toes utilization. Which of the following figures correctly depicts the interactions at the laptop Ron, when lack toes is not being utilized on the right, we see a figure depicting the lack opera and the control elements involved, and we see that lack see is going to be part of our lack opera. And the question asked us what will happen when the, uh, what will happen to the lack opera on when lactose is not present or not being utilized? So we have to look for the situation where lactose is going to be unavailable. And there are two situations one where there is high glucose and one where there is a low glucose. But in either case, the same thing that the same thing will happen to the repressor protein, and that is specifically what we will be looking at for this question. So on the left hand side, we see a figure with our question choices and we are going to be investigating what happens to two of the mechanisms on the lack opera. We will be looking at what happens to the repressor and what happens to the people Emory's so we should know that the are in April. Emory's as depicted back in our first figure on the right hand side will only bind to the, um, promoter region when our repressor protein is not bound to it. So we can only have one bound at a time. Either the repressor is bound to the operator or the um are on a plane. Race is bound to the promoter. Both can't happen at the same time. So if we look at the two situations here, we see that our repressor protein is going to be bound to the promoter region. Oh, are to the operator region and this is because the Professor protein will only unbind in the presence of lack toes. So our repressor plus lack toes means that our repressor is going to be released only in this situation. So when the repressors released our art, April embrace combined. So given this sequence of events, we can look back at her answer choices to determine what happens when lack toes is not there. So choice A shows us what happens in the presence of lactose, so it will not be the correct answer. And choice be shows us that our lack toes is bound to the repressor. And we know that this is incorrect, because if lactose is bound to the oppressor, they should be doing what it is doing in choice. A. So that is also incorrect. Choice. See shows that the are in a preliminaries is bound to the operator region, which we know will be incorrect based on the fact that, um, we know that our repress or in this case should not have lacked was bound to it. And us should still stick to that operator region, meaning that aren't a primaries should not be able to bind. And finally, we have our choice de, which has the repressor but with an empty slot for lack toes. So there's no lack toes bound, which is correct if lack toes is unavailable. So Choice D is going to be the correct answer


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