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List what DNA intercalating dyes can be used for qPCR....

Question

List what DNA intercalating dyes can be used for qPCR.

List what DNA intercalating dyes can be used for qPCR.



Answers

List the substances required for replication of DNA catalyzed by DNA polymerase.

So the substrate for D N A replication is going to be known as the oxy rye bone, nucleoside tried phosphates or, in simpler terms, D and T peas. It's cheesy, okay? And at the base is going to be a wry bos sugar or a deoxyribonucleic sugar. So a regular rival sugar would have Oops would have a hydroxy group at the two carbon and the three carbon. But the D accede rivals Sugar only has a head drunks a group on the three carbon and then go out here to the five private of five. Carbon is going to be attached to three. Or try phosphate groups. Three phosphate groups do those in red p there is one phosphate group is going to be connected to their phones, figures to that oxygen, and this one is going to be connected to one last pas. Vigor through the oxygen. Okay. And then on this side, this is going to be attached to a base from the one carbon. This is going to be a base, and the basis can be Adam ing Wani. If I mean or side of scene. And that is are the four substrates

She here office. First of all, describe the difference between SNP's and I'm str polymorphism So first of all with S and P's, So S and P's are known as single nucleotide polymorphisms, so there's essentially going to be a difference in a single nucleotide. In this particular case, well, str is there going to be short tandem repeats where it's going tohave repeats of sequences of DNA throughout the body so these could be used as markers as different people have. Different rates of mutation will have different mutations, generally speaking, so actually everyone's you meet different and this is the only way we can particularly be accurate in terms of this.

Okay, So how did scientists find out that DNA is genetic material? So we're gonna look at the Hershey Chase experiment. So her she chase knew about this T to bacteria phase, which which is a virus. They did not know which part of the T two was the genetic material. So the T two, they know, was about 50% protein, which is going to be this red and was 50% DNA, which is going to be this blue. So what they did or what they knew. Bacteria phases do they attach to a cell? Let's say this is E. Coli. They worked with the E. Coli touches to the sell. It injects this genetic material. It uses that genetic material and the cell to produce little offspring viruses, um, the offspring viruses. And then it explodes. We're not explodes, but it breaks free from the cell, and then it can go and attack other cells and reproduce and keep going that way. So what they knew is the new protein was the red and protein contains sulfur. So what they did is they used radio. You're radioactive isotopes of sulfur, and they grew E. Coli. So let's say this is a little echo allies. They grew E. Coli in this radioactive isotopes, so they know all of the proteins are gonna have this radioactive isotope that they can then view. And they know that the genetic material came from the protein because it was radioactive. Similarly, they did the same thing with phosphorus as we know from nucleotides contain phosphorus. So they grew these E. Coli in radioactive isotope of phosphorus. So they knew that if the if the tea to came in and it injected it's DNA in here and it replicated, they would see the replication, the replicated cells or the replicated T two would have this phosphorus. So eventually, what they found out was when the when they check to see the radio activity of these new T to after they had invaded the E. Coli, they did not find any sulfur radio activity in the new T to bacteria phases. However, they did find in the phosphorus they found that the T two bacteria faces When they reproduced, they had this radioactive isotope, so they knew that since the E. Coli already contained radioactive phosphorus in their genetic material after the T two was, uh, the new teacher were generated. They knew that the phosphorus was three genetic material, and therefore it must have been the DNA was genetic material.

So this question, once assisted, find substrate traveling and describe why it is beneficial. So substrate channeling is a process through which an intermediate is generated at one end of an enzyme is channeled to the next active site in the enzyme without ever leaving the end time substrate. Channeling has been official because it improves enzyme efficiency, and it does this by protecting certain formed intermediates from hydraulics ISS, and because of the intermediates, never leave the enzyme. It also prevents intermediate loss due to diffusion.


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