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In measuring long-term glucose levels in the bloodstream,glycated hemoglobin must be separated from unmodified hemoglobin todetermine the percentage of glycated hem...

Question

In measuring long-term glucose levels in the bloodstream,glycated hemoglobin must be separated from unmodified hemoglobin todetermine the percentage of glycated hemoglobin. Suggest asimple chromatographic method by which this separation can beperformed.

In measuring long-term glucose levels in the bloodstream, glycated hemoglobin must be separated from unmodified hemoglobin to determine the percentage of glycated hemoglobin. Suggest a simple chromatographic method by which this separation can be performed.



Answers

The iron content of hemoglobin is determined by destroying the hemoglobin molecule and producing small water-soluble ions and molecules. The iron in the aqueous solution is reduced to iron(II) ion and then titrated against potassium permanganate. In the titration, iron(II) is oxidized to iron(III) and permanganate is reduced to manganese(II) ion. A 5.00-g sample of hemoglobin requires $32.3 \mathrm{~mL}$ of a $0.002100 \mathrm{M}$ solution of potassium permanganate. What is the mass percent of iron in hemoglobin?

Two. This question is about, you know, being and, uh, it has 0.34 7% high on process composition, and each time ago, been molecule contains for hire. So you have a or higher in each Magog America. So the question wants us to calculate a golem ass off him above it on, uh, what we need to know. I used to know the mass off iron. I honest 55 to the Mass of Dionysus. Hi. Honest. Used to five boys age for five. All right, Um, it must have been making on my spleen. Six so high on this. 55.845 So if we multiply that by fall So 55.845 just by find it for five. Much fly by far because we were told that the monkey loving contains four iron atoms. So 55.45 months of life like fall when he waas too to read 0.3 Hates Grams said this is a total mass whole higher in the movie of being monocle. And we had heard that the person composition how higher? 0.3 force there. So that makes the 2 to 3138 grams divided by hex. We are X is the total mass off the program. You know, the small amounts of hemoglobin to fly by 100%. This because to 0.3 or 7%. So we make hex the subjects of the formula. And now he wants to actually forced to soon to 3.38 much fly by 100 percents Mrs. And grabs well, the love their 0.347% on now be because 264374 on 63977 Gramps. So that is the the mass hot er molto been, which is also a costume of the mamas Because that is, uh, uh, more nickel. Well, I'm

We're going to Kohli the movie. Maso. Hema Hoping like even that. See report 347% off the mass off he will go. Beings aren't any continued for I an atom. All right, so first off or we are going. Teoh, look for the massive firing. So with an ass off iron, you see close to 55.85 grand, Permal All right, But I didn t mogul being We have a four, so we'll go looking at 44 I am So we have 35 25 times for And then we have 2 to 314 grand, Permal. All right, so soon We're already know the, um master stand age. So say we have to move the mass off. Ah ah, hemoglobin. And I was just quite human time. 0.3747%. And you should be close to the massive for, um are in atom. So it should be close to 22314 So 2 to 3 14 grand for mo. All right, so then sure Black. Ok, so we're going to solve for this. Ah, equal insurance. There were 2 to 3.4 divide by 0.347 percent and we should be able to see it as a course of 64 380 grams per mole for the murder mass off him ago plane.

Given that hemoglobin is .35 iron biomass, let's determine the iron atoms in a hemoglobin molecule. The percent of iron is equal to be massive iron, divided by the molar mass of human myoglobin Times 100%. Given that the % is .35%, iron are unknown. Here is the mass of the iron And were given the molar mass of Hemoglobin. A 64 458 grams promote Comes 100%. And solving for the massive iron, we find that it's equal to 225.6 grams per mole. So let's find out how many iron atoms there are. Yeah. Mhm. Starting from the long mess Of the iron here, 55, 8 g. Uh iron in one mole of iron And in one mole of iron, we would have 6.02 times 10 to the 23 iron atoms. And this would be equal to 243 times 10 to the 24th iron atoms that would be present in he uhh hemoglobin molecule or promotion. Mclovin

So this problem deals with hemoglobin and for part A. You want to find its molar mass. So you know that the chemical formula for human woman c 2952 h 4000 664 and 812 oh 832 if eight and F E for so. First, we have 2000 952 carbon atoms, each of which are 12 point no. One ground from all 4664 hydrogen atoms, each of which 1.8 graphs from Rome, 812 Nigerian Adams, 14.1 grams from all 832 auction Adams, each of which are 16 grumps more eight sulphur atoms, its atomic mass of 32 point 07 And then finally four iron atoms such as an atomic mass of 55 0.85 So when you add all these together, we get that are mullah mass of hemoglobin 6.5 32 times 10 to the four grams Promote now for part B For part B. We want to find the mass of hemoglobin and every human adult, and so were given a couple of youthful numbers for to solving this problem. So we know that every human has five point of leaders of blood and that in every mill later blood, we have 5.4 times 10 to the nine red blood cells. So permanent leader, and then finally, we have two point eight times tend to the eight hemoglobin molecules, chemo molecules for every one red blood cell. So those are gonna be are useful conversions when we're gonna find the final answer. So we know that every human that we're gonna start with has five point leaders of blood and you're to convert this to middle leaders, so we just multiply by 1000. This is where our first number comes because we know that for every middle leader blood But there are 5.0, terms tend to the nine red blood cells. And then our second conversion comes in, you know, they're 2.8 times 10 38 hemoglobin molecules for everyone. Red blood cell. The next up we need to do is we convert this two moles of hemoglobin because right now we have molecules of human woman so well, just divide by Abu Godric's number, just six point over two times 10 to 23. You know that that's how many molecules you are in one more of any substance, and I would get moles of hemoglobin. And finally we just multiply by the more massive hemoglobin that we calculated per a just 6.532 times 10 to the four grams for every one more. I'm hemoglobin and in the most play all that out. You will get that the molar mass or not, the molar mass, but the total mass of hemoglobin and every human adult. There's 7.6 times intentionally two grams. That is a final answer.


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