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Imagine you're a scientist characterizing a newly discoveredmembrane channel protein. What two observations might you make thatwould demonstrate to you that th...

Question

Imagine you're a scientist characterizing a newly discoveredmembrane channel protein. What two observations might you make thatwould demonstrate to you that the channel protein conducts active,not passive transport. For full credit, explain how theseobservations allow you to make your conclusion.

Imagine you're a scientist characterizing a newly discovered membrane channel protein. What two observations might you make that would demonstrate to you that the channel protein conducts active, not passive transport. For full credit, explain how these observations allow you to make your conclusion.



Answers

Which statements are true? Explain why or why not. Transport by transporters can be either active or passive, whereas transport by channels is always passive.

Yeah. Okay. So four problem. 35. What we want to think about is the differences between a protein that resides in a membrane and a protein that's in a water soluble water soluble area. Right. So some buffer. Right. I don't know how many of you have been on the biochemistry lab, but obviously we're going to have a buffered solution that are protein is going to be rolling around it. Okay. Um on the left we have a situation where we have a protein and a membrane on the right, we have soluble protein. Okay, so we can think about the fact that on the internal part of our soluble protein, this is where we're gonna put all of our non polar residents. Okay. Or non polar residues are essentially going to be hiding from the water. Yeah. Yeah. Coming out. Mhm. Towards the water. Yeah. Is gonna be your polar residues. Yeah. Yeah. So just remember like, dissolves like and like associates with like, okay, non polar is gonna be hiding from the polar. Are polar residues are going to be out interacting with the water. This is the case for a soluble protein. Okay so one that is in a water environment whereas here what we see in our membrane is that the internal part of the membrane? It's actually non polar. Okay so that means the inside here is actually going to be where your polar molecules are going to be. So your polar residues whereas interacting more so with the membrane itself is going to be your non polar residues I guess. I'm just gonna put N. P. Thank you. Okay okay so just a reminder of this you've probably heard a million times at this point like dissolves like when we think about interactions of proteins like interact with like okay so the internal here will be the polar molecules interacting with each other or as here are non poehler's would be interacting with each other and that would be the exact opposite for a protein that is in a water. Sybil. Yes. Okay.

The between. So a boat. The programs that decide in the non polar in the year. Okay, membrane fold. How will they behave now? Those proteins, proteins, buildings that reside Indeed, no water interior off the membrane food, they will fall. Actually, this these proteins will food so that it's known for a residues Are yeah. New polar residues on the outside of the protein in contact with no horror membrane. Oh, and polar residues arm inside the proteins. Internet. So if you have a memory, the delivery there is no for us membrane. So this area will be consisting. No foreign groups, no water residues on dwelling said all other cities.

So they're talks a carrier proteins to be a simple affair. Anti pulled in in a news reporter. So with us imported, this works in a positive. I'm brilliant and it would choose, but molecules across the cell membrane at the same time. And anti porter transports to our normal feels that opposite direction. So one molecule go in this out on the other molecule. Wiggle out of this and a unicorn takes one. So sends a music in one direction. So two of thes can mediate secondary active transport. And these are the simple various van 20 porters, and this is because it can you molecules against the concentration reading.

Well, this question a polar molecule and his concentration outside yourself okay is higher, then inciting itself. So an. In addition, the concentration of people a molecule is gradually increasing within the cell. This means that the Polamalu kill is, um Griffin A games. It's concentration. Radiant. This requires energy. In my defense, the molecule is pulling nature, which means that it cannot bomb the feels into the cell. We got the help off looking China. So if this information, our best hypothesis for the process that we are observing will be a day the polar molecule is driven into the cell by the means off an active transport process. So the situation


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