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1. A. What is being detected in this assay to measurecaspase activity? B. If high caspase activity is detected in the cell, what wouldthis indicate?2. Why is AcDEVD...

Question

1. A. What is being detected in this assay to measurecaspase activity? B. If high caspase activity is detected in the cell, what wouldthis indicate?2. Why is AcDEVD-AFC included in the reactionmix? 3. Other than the caspase activity assay, list one other way youcould experimentally test for caspase activity.

1. A. What is being detected in this assay to measure caspase activity? B. If high caspase activity is detected in the cell, what would this indicate? 2. Why is AcDEVD-AFC included in the reaction mix? 3. Other than the caspase activity assay, list one other way you could experimentally test for caspase activity.



Answers

What simple chemical test could you use to distinguish between the members of each pair of compounds? Tell what you would do, what you would expect to observe, and how you would interpret your experimental observation. (a) Pentanal and 2-pentanone (b) 2 -Pentanone and 2 -pentanol

How can we distinguish between and Zelda Hardin Cycle picks alone and seats all the highs and acid. Um, so in both cases, we have an multiplies and Akita, and we can distinguish between these recently with Collins regions. So we asked ones Reagan's on and this and also heights, it's It's over, Mira, whereas keystones is no so that okay, enzymes height and give us a mirror of the Group B A C tells highs. So that's what keeps Evans with.

When Phoebe ought to, we we go to react with conch agent 03 When PB and not to react with HMO three. So here the product bombed age, U PB And a three hole, too, plus two as to plus or two. So here or to guess it's produced an itinerary accent aged It is a reaction the for Metal Islands Metal Irons. So option age, correct and said for this problem.

So moving on, we are just looking at similar infrared spectroscopy. So another the stretch of a C H bond. Often I'll the hide containing molecule has two absorption peaks. So if the reaction had occurred, the intensity off these absorption bands would decrease until they were no longer present on our spectrum. So the product of the reaction here is missile benzene. If the reaction was to have a card of the absorption bands, 2960 to 2850 wave numbers would be present on the spectrum due to the S P three c h is that is on Army Thel substitue int. So the stretching vibration for the NH functionality occurs at 3300. The bending vibration for that same functionality is slightly lower at 1600. Therefore, the N H two and H two hard in fact, been removed. The previous two absorption bands that we discussed above would not actually be present on the spectrum

All right, So we want to know which of the following tests is best used to determine whether or not a disinfectant has been used properly. And so your answer is going to be see the in use test. So what is the in use test? Well, basically, you take but this infect int that has been used in a clinical sitting right, and you combine it with some sterile broth that also includes a compound to deactivate essentially the disinfectant. So you combine those two and take a few drops from the combined solution and add them to place, and the plates are incubated under different conditions. So you have two plates. And if more than five colonies grow on either plate, you know you have had some contamination in your disinfectant, and that's how you know it wasn't being used properly surrounds her seat


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