This'd is a problem. Looking at a restriction map of the second of DNA and ah, drawn it out here and it shows you a segment of DNA A. The numbers underneath represent base pairs. Eso there from this mark from position one to position to. It's 5000 base pairs from position two to Position three is 3000 from three before is 4000 from 4 to 5 is 2000 and 5 to 6 is another 5000 base pants at positions 13 and five. We see the word echo E CEO, and that is representative of the ECO R one restriction enzyme recognition site. So this means that position one Position three and in position. Five. The enzyme would be able to cut at those places because they're the regular recognition sites or presence. PST represents the recognition site for a restriction Enzyme PST. One. Those recognition sites are at position to position for position six. Finally, the other piece of information we need to know is that are pro, which is represented here in blue would overlap with this piece of DNA, so it overlaps starting here and ending over here. So now we're going to look at different patients, D N A. And we're going to cut them with certain enzymes and then try to find the pieces that will Aneel or hybridize to our pro. So in the first scenario, we have a paint patient. One patient one has all six restriction enzyme sites. And they're saying that you are digesting this DNA with the inside PST. So we're going to digest its site, too. Site for insights. Six. Now we add or pro. And the question is, what size pieces will Aneel toe are pro. So since we're going to have a DNA cut at Position six and position for this will be this problem, it will be 7000 base pairs. Since it overlaps with the probe, 7000 base pairs are going to be one of the, um, pieces of DNA we find, and we're going to cut from position for to position two, and we're going to create another 7000 base pair piece, and it too, will a meal to the pro because there is overlaps. So for this patient, we will get to pieces that are approximately 7000 base pairs that will a meal to the quote. The next question asked about a nen vivid jewel patient to impatient to has a mutation that eliminates position for so they do not have the recognition sequence for this enzyme PST. All right, what happened to me to adjust with PST now? Well, we don't have this site ending over, so we will cut at position two and we will cut a position. Six. So that means we will create one piece of DNA A this length, um, which will be 3000 plus 4000 plus 2000 plus 5000 which is 14,000 base pairs. And it will bind to this pro. And so for this patient without position of without the recognition site at position number four, we will create one piece of DNA that is 14,000 base pairs long that will bind to the pro next individual. Individual three has a mutation that eliminates positions to spy. So they do not have this, uh, echo or one recognition site. What happens if you digested with PST again? So it really doesn't matter that they're missing the site, the PST sites will be recognized. And will the DNA will be cut so we will get a piece again from this set from position sixth position for which will leave us with a 7000 based therapies. And we will have another piece from position for to position two, and that's 7000 base pairs. So we will have two pieces of DNA at 7000 base pairs that will a meal to the pro. Next question is back to, um, patient one. So they have all of the all of the enzyme sites. But in this case, now we're digesting them their DNA with both PST and eco or one what will the pieces look like? So once we digest this DNA with all with both of these enzymes, we're going to create a piece. Uh, we're going to get a cut. This piece will be 5000. These fears the next piece will be 3000 base pairs. The next piece will be 4000 base pairs and next piece will be 2000 days. Pierce and the next piece will be 5000 base pairs. Those will be produced from the inside digestion. But the next part of the question is which pieces will actually a meal to our probe and the Onley pieces that will a meal with to the probe here will be those that overlap sequence. So in this case, it we digest with both enzymes and then a meal with the probe. Who will get the 4000 base pair piece. We will get a 2000 base pair piece and we will get a 5000 base pair, please. So, finally, the last part of this question asks us about another patient. Who, um so patient five. Excuse me. Patient three. That has. Sorry. Patient three, but he has no, um site at position five. So position five site is missing. Get back to you. So, positions, position five site is missing. So the question is it we digest now with all enzymes. What will we end up with? So we will not. We will not have position five cutting, so this will end up to be one large piece. So this will be a 7000 base pair piece and we will have a 4000 base pair piece that will also a meal. So in the last scenario they give you, you will end up with a 4000 base pair peas as well as a 7000 base pair, please. I think what's really important in this problem is to realize that, um, just get out of this is to realize that the Onley pieces we care about are those that will kneel to or pro. So Onley pieces that overlap with our pro will show up on our job because the probe is going to a Neil toe on Lee, the pieces that have common on sequences.