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I need PDA test result for Syphilis.In PDA Test for Syphilis. What are the possibleoutcomes, and the organism(Treponema pallidum) expectedresult.Example of physiolo...

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I need PDA test result for Syphilis.In PDA Test for Syphilis. What are the possibleoutcomes, and the organism(Treponema pallidum) expectedresult.Example of physiology test: CatalaseThe following is an example of what is expected for the synopsisa test, using Yersinia pestis as the organism.Catalase is an enzyme that detoxifies hydrogen peroxide, whichis a toxic form of oxygen, into water and gaseous oxygen (Leboffe& Pierce, 2016). If an organism contains catalase it will beable to survive in

I need PDA test result for Syphilis. In PDA Test for Syphilis. What are the possible outcomes, and the organism(Treponema pallidum) expected result. Example of physiology test: Catalase The following is an example of what is expected for the synopsis a test, using Yersinia pestis as the organism. Catalase is an enzyme that detoxifies hydrogen peroxide, which is a toxic form of oxygen, into water and gaseous oxygen (Leboffe & Pierce, 2016). If an organism contains catalase it will be able to survive in the presence of oxygen. This test can be performed by adding several drops of hydrogen peroxide into a freshly grown slant. It is important to cover the test as soon as the hydrogen peroxide is added to prevent splattering from a positive result. The break down of hydrogen peroxide is evidenced by bubbles, which is the release of oxygen, thus designating the organism as catalase positive (Leboffe & Pierce, 2016). If there are no bubbles, this indicates the absence of catalase and the organism is considered catalase negative. According to Microbiologyinfo.com (2020), Yersinia pestis is catalase positive.



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Investigators need to determine which of 600 adults have a medical condition that affects $2 \%$ of the adult population. A blood sample is taken from each of the individuals. a. Show that the expected number of diseased individuals in the group of 600 is 12 individuals. b. Instead of testing all 600 blood samples to find the expected 12 diseased individuals, investigators group the samples into 60 groups of 10 each, mix a little of the blood from each of the 10 samples in each group, and test each of the 60 mixtures. Show that the probability that any such mixture will contain the blood of at least one diseased person, hence test positive, is about 0.18 . c. Based on the result in (b), show that the expected number of mixtures that test positive is about 11. (Supposing that indeed 11 of the 60 mixtures test positive, then we know that none of the 490 persons whose blood was in the remaining 49 samples that tested negative has the disease. We have eliminated 490 persons from our search while performing only 60 tests.)

All right, All right, question 90. So the question that the students asked is how Well, uh, ph influences enzyme activity. Uh, so the activity basically means how well can an enzyme work at certain conditions? Maybe it works better in certain temperatures, or maybe better at certain pH levels. And that is what the students are testing. And specifically, they're testing the enzyme that is made Natalee's. And Catalyst basically helps a reaction go through to completion students lists. Hi, Jim peroxide. And this is just water with an extra oxygen molecule in. Catalyst can basically take this enzyme. Put it through a reaction into, uh, to water molecules and oxygen molecule. And that's what happens when the enzyme is able to work on hydrogen peroxide. Uh, test tubes six through 10 had water. Put it in them. They didn't have Cavalese. So all the others one through five, they all had cattle stands on, we're looking at, but 6 to 10 had water, and this was added simply because they needed a control just to see that none of the test tubes were tampered with or there was no outside influence. Just to know for sure that yes, catalyst was the only thing acting on the 100 peroxide. So these are all control, and it's actually good that there were no bubbles in it. So this is actually good. And why is that good? Well, bubbling was actually oxygen molecules. So if you see an oxygen molecule, that means this reaction went through. Uh, that means the cattle descends. I'm took this. Hydrogen peroxide made it into oxygen that was in the form of air bubbles. So air bubbles, air good air bubbles and me, and the reaction was carried through successfully. So since test tubes 66 to 10 were controls when we tested, Yes, there is no bubbling there. Good. We don't have to worry about them. For the rest of this problem, we're just gonna focus on test tubes one through five. So mount bubbling again means oxygen was produced. So we want to look at the most amount of oxygen to determine if catalysts works the best. So looking bubbling, we got none for Ph one. That means that ph one, the enzyme just wasn't working. Well, it was way too acidic to actually, uh, have the enzyme work, so amount of bubbling for three and nine. PH is only one plus sign, so that's not very good, either. It's working across those out, and lastly, the Ph five and seven uh seven had five plus science. Ph five had three. That means it was way more bubbling and ph seven, which means testing for with three milliliters catalysts. Uh, what's the the best in terms of enzyme activity is actually goes coincide with mammals. So a lot of mammals are aerobic and yeah, pH of humans, I believe, goes from 6.5 to a point. Oh, so that's seven actually coincides alive and makes sense based on common knowledge.

So we're continuing on with our DNA topic on. We'll jump straight into the content here because we've already looked at some background basic information. So the first thing we're taking look at here is purified water. So the play is treated with this purified water that acted as the negative control in this given experiment. Because the water is not a muted gin, so it will not result in any sort of reversion, and therefore the number off colonies observed will be, in fact, the minimum number on represent false positives. So all of the mutations must be compared with the negative control plate before we start to interpret results. The second part we have are known Musa Jen. So the plate containing the known mutation acts as our positive control in the experiment. So, um, you to general caused the reversion on the growth of colonies on the play itself, which will provide a basis for comparison off unknowns that we've tested. Sad part we have is the experimental sample to the experimental sample. The sample that's tested shows a very small amount off growth of colonies, and this is similar to the water negative control play that we discussed in the first part, so this indicates the absence off the reversion upon treatment off salmonella with the compound. Therefore, this test to compound seems to like the youth and the Genesee. So any final part we have to consider the predominant function of the liver is the metabolism off toxic molecules in the body as well as other different molecules by its enzymes. So these metabolic reactions tend to result in the detoxification off compounds, which makes them harmless to the body. However, in some cases the metabolic reaction makes a non toxic compound toxic, which would not be a good thing. So, for example, in the Ames experiment, the chemical tested in place, see was retested after the treatment with liver ha Mancini on, we see a large number of colonies observed on the play itself. This indicates that the extensive reversion it induced by the chemical of the process in the presence off the liver homogeneous upon the plate. So this is indicative of the fact that the chemical, upon treatment with the liver homogeneous eight, was converted into a very mutiny genic compound. So this is the opposite of the sort of reaction that would be looking for the liver to carry out. So a comparison of this plate where the positive controlled also further supports the idea that we do have a mutiny genic compound upon the plate now.

No question on you three asked which organo is closely linked hydrogen peroxide and I've gone ahead and written out the formula that was given in the previous questions as well as the multiple choice answers. So, um, we're going to do is just look through each option out and figure out which one is the best one. So the first option is in nucleus, and we have toe think back on what the new Griest does. And so the nucleus is, um, the organized stores, genetic information. And because of that all, it's not likely that hydrogen peroxide would be linked to that. And so we can go ahead and cross that out as well as Option C nuclear list, which is a part of the nucleus, um, and then be Riva Zone. So Riva Zoom is responsible for protein for, um, creating proteins survive assumes, create proteins. And so again, that wouldn't really make sense. So we can go out and cross that out, and then the Golgi body is responsible for altering and, um, fixing up proteins and live. It's and that really they wouldn't make any sense to have any hydrogen peroxide linked to that and So the only thing we're left with is a mitochondria. And mitochondria is like what people like to call the powerhouse of the cell because aids inside of the restoration. And it creates, um, energy from, um, proteins. And so, um, if we look at this answer, it makes sense because, um and so restoration, you need oxygen. And so that's probably the best answer given the off mission that you have from the previous questions, as well as from the formula.


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