Using the following sequence, illustrate the process oftranscription by filling in the correct bases forthe mRNA.Sense: A C G G G G C C T A CATemplate: ...

(a) Write the sequence of the mRNA molecule synthesized from a DNA template strand having the following sequence. $$5^{\prime}-\text { ATCGTACCGTTA-3 }^{\prime}$$ (b) What amino acid sequence is encoded by the following base sequence of an mRNA molecule? Assume that the reading frame starts at the $5^{\prime}$ end. $$5^{\prime}-U U G C C U A G U G A U U G G A U G-3^{\prime}$$ (c) What is the sequence of the polypeptide formed on addition of poly(UUAC) to a cell-free protein-synthesizing system that does not require a start codon?

Okay, I am going to write the complementary Strand Messenger or in a strand, the messenger or in a in a 5 to 3 direction. And that's going to include its code on sequence and the amino acids that it encodes. Or so let's first do the complementary strand for DNA. The DNA strand that was given runs in a 5 to 3 direction. DNA's anti parallel to the complementary strand runs in a 3 to 5 directions. I'm going to start on this side now. The ad mean always piers, but the timing and the side of seen always piers with the Guan E. Right now I'm going to write The messenger are in a strand, just going to go 3 to 5. But I'm going to write it 5 to 3. Now, this is going to complement this original strand and look at the similarities between these two strands. So dream Okay, A we do know have finding a messenger or in a so it would be euros ill. All right now I'm going to switch this messenger or in a to a 5 to 3 prime direction, um, with the code owns so code owns air going to be sequences off three. So we're going to flip this around so we're going to have cheating a a You see, she and C G you now I have divided that into its code ons, which again are going to be three of those bases and then the amino assets that they cope or you used the genetic code and this would be glue tannic. Pass it. This one would be Cering, and this code on would curve for arching.

Thats question below is a DNA sequence in vision that this is a section of a d n a molecule that has separated in preparation for transcription. So you are only seeing the Yankee sent strand construct the M R in a sequence transcribed from this template. So first we have to distinguish between the anti says and the since Strand's So the antisense strand is going to be our template strand, and our sense strand will be our coding strand. So right here we're given our anti sense. We're template strand. This means that we will first have to construct the coding strand of DNA. So as we know, DNA is going to be synthesized in the five prime to £3 of direction. And, um, this way there, there are two options that we have. We can either construct the DNA. Ah, that would be complementary to the template. So the coding strand where we can just go straight to the m r and a. But just to get an idea of what we're doing, we're going to do that middle step of, uh, constructing the d n A strand first. So remember when we're, um, looking at DNA nucleotide disappearing. We know that a present T and C pairs with G. This is for DNA. So since we're looking at our coding strand right here of DNA, then we will follow this base pairing rule. So we will have a T g a C T g a C T g c T a G. But now we have to convert this DNA into M R in a. And this will be our coding, Marnie. So, uh, to do this, we will once again have to flip. Uh, we will base this off of the coding strand. So since we started off with our template strand, we simply to get our m r name, we have the same £5 of three prime orientation because oh, that we're doing is we're substituting in for every t that we see, we're going to substitute in a you because for Arnie A's pairs with a pairs with your so or you and see pairs with G. So this is the are in a nucleotide base pairing rule. So we just take what we have from our DNA, and we're going to, uh, copy it exactly, except for every t that we see we're going to replace it with you, so we'll have a you g a si you g que si u g c you a g So this right here will be your answer.

In this question. We're looking at this strand of DNA and part a asked us to rate the complementary sequence five prime to three prime. So I'm gonna start off just by writing it underneath the strand and matching it up and the multiple bit So it's five grand a year, Brian. So this is the third time to five point version. So T pairs of A and C pers g b c a t c c t a five primes over writing it. Five prime to three prime. Start here. Well, just flip it around. So a t c c t a c she a Part B asked us to write sequence or the strain of m R and A that would be complementary to that original strain of DNA. We're also writing this five prime to three prime. So the only difference between the complementary strand of DNA on the complement of RNA is that we're replacing T with you. Since you are in a uses, you're still, instead of finding that would be a U. C. C you a c g. A. Part C says that assuming this sequence is part of the coding sequence for a protein and that it's lined up. So the first coat on here is the five prime. Um, they asked us to write the sequences for the free anti code ons that would be complementary to the strand of em are on a So let's right, the compliment first in purple. So that would be you. A g g a u she see you. Um, and that is three prime five prime. But we're gonna write the code on by a primitive three prime. So the first one would be g u A. And then we have you a g and you see, g and then part D asked us what sequence of amino acids and selected by this m r and s o. Here. We're still talking about this Amar and a up here. Um, and, uh, What? It tells us that you can use any chart on the Internet that you'll find for the complete genetic code. So, um, a, you see is the first code on here and then see you a and then c g a. So a You see codes for isil. Lucy. Oops, So losing. See you. A codes for losing and c g A codes for Argentine. So I still losing. Losing Argentine would be the sequence of amino acids here

This question involves the topic of base pairing and the general process of transcription. First. Let's see about about base pairing. Remember that you have for D. N. A. You have four naturally no spaces adding. Okay, You have one in, you have cytosine and you have saving for R. N. A. For any type of RNA. The only difference is that instead of timing you have in your arsenal. Okay, that's the only difference. Now. Remember that happening piers with timing or your asylum case of RNA and wanting bears with cyrus in. Now let's talk a little bit about. A DNA. DNA is made of two cents. Okay, this is going to stand and this is the other stand. It just has a name. For example, I suppose this instant is the cold instant according to stand and this is the template strand. The coding strand is called like that. Because when this DNA undergoes the process of transcription, it means the process by which uh M. R. N. A. Is produced. The M. R. Name is going to have the same nucleotide sequence than to the coding standards A. D. N. A. It means if the coding system has a has a following sequence of A then the the M R. N. A is going to have the sequence A. Two. If it is a G C. T then it is going to be G. C. You remember that you use your brazil instead of timing in the in the RNA. Okay, so that's the coding stand talking about a temporary stand. The temporary sent as its name says, it is useful as a template for transcription. So for example and it is going to have the complementary sequence than the M. RNA. For example. Let's give another example. Not this. Let's suppose the template is A C. G. D. A. Okay, so what will be the sequence of the M. RNA? As I mentioned, the template has the complementary sequence to the M. RNA. So the marinade, it's going to be that what binds what place a dining remember it pairs with uracil? So they're going to be in uracil uracil, G C. E. In you. It is going to be the aymara name. Okay. And if you want to know that coding a son for DNA, well it is going to be this sequence. But remember that with time instead of curiosity, it means that calling a son would be T T G C A. T. Okay, so with this knowledge let's transcribe this sequence. Okay, this is the first sequence. Um The question says that all the sequences they are providing us are the template DNA sequences. It means this is the complementary strand to the marina. So our marina is going to be the complementary strength to this. It means instead of tea it is a instead of G it is a C. Instead of a. It is you you the G a do g c c a U G G and you Okay. This is the M r n A produced from this template. A strand of the DNA. Now let's subscribe the next DNA sequence. Okay, as this is the template, the stand then the nitrogenous base that pairs with G. Is see the original space that with C. S. G. A nitrogenous base. That there's a your A C. The G A U U U. She U a n G. Okay. This is the M RNA produced from this is simply the strand of DNA.