5

1. Determine the number of bacteria per ml: of water specimen_Determine the number of bacteria per ml; of water specimen: Jmi IMewater specimcnH,o blanks9ml9 ml9 ml...

Question

1. Determine the number of bacteria per ml: of water specimen_Determine the number of bacteria per ml; of water specimen: Jmi IMewater specimcnH,o blanks9ml9 ml9 mlamount platedpour platc counts'46145Determine the number of bacterial cells per ml: in the original cultureggml99mnggm[g9moriginal specimen E: coliIml0,Iml Iml0.Iml Iml0.Imltoo many to count >500 128 colony counts

1. Determine the number of bacteria per ml: of water specimen_ Determine the number of bacteria per ml; of water specimen: Jmi IMe water specimcn H,o blanks 9ml 9 ml 9 ml amount plated pour platc counts '461 45 Determine the number of bacterial cells per ml: in the original culture ggml 99mn ggm[ g9m original specimen E: coli Iml 0,Iml Iml 0.Iml Iml 0.Iml too many to count >500 128 colony counts



Answers

If the concentration of $E .$ coli in a broth is between $10^{4}$ and $10^{6}$ cells per $\mathrm{mL}$, the best way determine the precise number of living cells in the sample, would be to a) use a counting chamber. b) plate out an appropriate dilution of the sample on nutrient agar c) determine cell number by using a spectrophotometer. d) Any of these three methods would be satisfactory. e) None of these three methods would be satisfactory.

In this problem where asked how we would approach counting a sample of E. Coli that was cultured in broth and it lets us know that the concentration is 10 to the 4 to 10 to the sixth cells per mil leader. Our first task here is to exclude any counting methods that don't apply in this situation. So first we check can equally be cultured in the laboratory and the answer is yes. Yeah equally is great for culturing in the laboratory. Next we consider are we trying to count living cells or living and dead cells? And in this case we want to count living cells. So when you want to count living cells for an easily cultured substance like E. Coli, then you're gonna want to use the colony count method. We can also double check to make sure that our concentration is high enough but we should be good. So how do we do a colony camp? Well first we need to take our solution of E. Coli and do a series of sequential delusions. Where we carefully mix a small amount of our sample with water so that we get fewer and fewer bacteria in each test tube. Then we can take these test tubes and smear them on an agar plate and try to culture them. And our goal is to culture a sample that is small enough when we spread it on a nutrient agar so that each individual cell is spread out and can form its own colony. Then we simply count the number of colonies and that tells us how many cells were in that deluded sample. And we can multiply based on our record of the delusion to figure out how many equal I were in the sample in total. To verify that this is the right method and our problem we can examine the other options. They also give us a suggestion of accounting chamber or a spectra Fatima Tower, which uses light to measure the number of bacteria. But the problem with these options is that they will also count dead cells. Because the light is going to pass through dead cells and living cells the same and the counting chamber, it's going to be really hard for the visualization to distinguish between living and dead cells. So because we want to count living cells, only our answer is B.

Should be statements about coliform testing is true. A. They all determine the number of E. Coli B. The M. P. N. Procedure precisely indicates concentration. See the media used checks with the ability to ferment lactose. See a positive test indicates that pathogens are definitely present. Or e all covert forms titlist om PG and mut. Okay let's start at the bottom. Do all color forms Hydrolyzed om Petri and energy. No nobody in it. Uh The reason we use these is because lactose fermenting bacteria. Hydrolyzed O. M. P. G. E. Coli does not E coli hydrolyzed MG lactose fermenting bacteria do not. So it differentiates between different color forms. Okay d does a positive test indicated perfections are definitely present. No this isn't true. It just shows that fecal bacteria are present. They may not be pathogenic. You may have completely harmless strains of e coli presence. No patterns at all that you would get a positive test. Yeah. Do all of them show you the number of E coli present? Uh No not necessarily. If you were looking specifically at O. N. P. G. You wouldn't get you wouldn't get a measure of e. Coli at all the the mpm procedure. So this is the most probable number methods and as the name suggests it is a use of probability. It does not tell you the exact concentration. It shows you the most probable concentration which leaves an answer as being see the media used to check with the ability to ferment lactose ups. And this is indeed true. If you have Alexis containing growth you can test um for the ability to metabolize galactose and if you get positive results then you know you have color forms present.

So Hello, strain. Now we're going to a national discussion, and this is about back to growth. The consideration out bacteria be in millions per millimeters x arses. So this is X are given. Wait 3.5 and even the power geo 0.2 X no, a upper two hours. So be toe skull. Collect the 20.5. The power Geo point. No. After one hour. So this is one so 0.0 through in one article collapse and you will find. And this is equivocal. Three wind pipe, seven 1,000,000 per millimeter. 1,000,000 part millimeters and be after 6.5 hours. Be 6.5 hours. Letter. It's a 3.5 and you topology upon Jiro. Go into 6.5. Which is, uh, 3.98 1,000,000 for Milliman on See Upper. How many hours? Well, they're six million bacteria. So bacteria the B x of the relation, which is 3.5 feet board 0.2 x. The solution is given and already six million bacteria. Harmony Arts. We're trying the X. So you'd about 0.2 x No, this 63.5. You'd apology to find zero to X now Taking Alan board side on This is Ln six minus 11 3.5 equal to geo plan General Who? X Island. It's l. A new one. Beautiful one No x equal to island six minus island 3.5 upon geo 0.0. So yet x comes don't miss six year, 26 parts.

So hello. Stand nowhere. Don't undressing this question this consideration are bacteria be millions but millimeters upper exercises given by B X. I was just given as 1133 and either power 0.15 x So first, how many bacteria? Over 2.5 hours. So be 2.5. You'll find 1.33 and feet about you don't find 15 in tow. Go find five pars little. So if you calculate this, Miss comes around for 1.9 to the fight Millions. But let me and be upper $8. So be a 1.2. Either our you'll find 15 Hey, on optical. Listen, it's abuse. 4.41 million minutes on See Khomeini are so it'll take 13 billion bacteria to be X equal. Given one point to the three and you'd apology you'll find one x No, but given 31 1.3340 point 15 packs. Now do I weigh 1.3? So 31 by 1.33 Corporate power. Do you find the 15 X thinking in love initial board say on the office. Not here Ellen 31 upon 1.33 equal to 0.15 x in tow. Ellen. So this gives uh Ln 31 minus Ln 1.33 upon. See, this is gives a little one up. 10 point Judo one fight, not Japan. Gero Gero points So genital point one type people do X so x Listen, get executed. Don't be ours.


Similar Solved Questions

5 answers
1. Roll an even dice and observe the number N on the uppermost face: Then toss a fair coin N times and observe X_ the total number of heads that appear N tosses _ Write down the conditional probability mass function pxiv(13). (ii) What is P(X = 5)? (iii) What is E(X)?
1. Roll an even dice and observe the number N on the uppermost face: Then toss a fair coin N times and observe X_ the total number of heads that appear N tosses _ Write down the conditional probability mass function pxiv(13). (ii) What is P(X = 5)? (iii) What is E(X)?...
5 answers
Poliovirus: Where is this pathogen found today?How do people get infected with polio? Do most people who are infected with this virus experience illness? After infected with polio, when could symptoms appear?What is the treatment for this disease?Describe the movement of this virus through different tissues of the human body:Why is polio , greatly reduced in most of the world today?
Poliovirus: Where is this pathogen found today? How do people get infected with polio? Do most people who are infected with this virus experience illness? After infected with polio, when could symptoms appear? What is the treatment for this disease? Describe the movement of this virus through differ...
5 answers
Short Ansnerpoint (5,12) and minimum at the point (12, [2 marks] sinusoidal graph has maximum at the 5) . Delermine the midline of the graph[2 marks] Determine the dcgree of this polynomial function List the propcrtics of the functionDelermine the degree, leading cocffcient; and constant tcrm of this polynomial function: Kx) 2 - Sx + 1Estimate thc value of 1729 in radians, t0 the ncarest tenth
Short Ansner point (5,12) and minimum at the point (12, [2 marks] sinusoidal graph has maximum at the 5) . Delermine the midline of the graph [2 marks] Determine the dcgree of this polynomial function List the propcrtics of the function Delermine the degree, leading cocffcient; and constant tcrm of...
5 answers
Question 154 ptsLet A= [EWhich of the following is equal to Ax?'~[H ~HJ 18 +2[2]+=[8]0 71 (1-2/+22 [2 ~2
Question 15 4 pts Let A= [E Which of the following is equal to Ax? '~[H ~HJ 18 +2[2]+=[8] 0 71 (1 -2/+22 [2 ~2...
5 answers
State il the uiengles In the pair zre cimilar lf s0 corrpkete the eimilarity saieentAABC ~Yes. they are similarNo, they not similarIf you answered "Yes" for the previous question; complete the similarity statement using only uppercase letters. If you answered "No" , type No" for this answerYourenswer2 State the triangles in the pair are similar If so, complete the similarity suatementLVUTYes, they are similarNo. they are not similar
State il the uiengles In the pair zre cimilar lf s0 corrpkete the eimilarity saieent AABC ~ Yes. they are similar No, they not similar If you answered "Yes" for the previous question; complete the similarity statement using only uppercase letters. If you answered "No" , type No&q...
1 answers
Compute the rank and nullity of the given matrices over the indicated $\mathbb{Z}_{p}$. $\left[\begin{array}{ccc}1 & 1 & 0 \\ 0 & 1 & 1 \\ 1 & 0 & 1\end{array}\right]$ over $\mathbb{Z}_{2}$
Compute the rank and nullity of the given matrices over the indicated $\mathbb{Z}_{p}$. $\left[\begin{array}{ccc}1 & 1 & 0 \\ 0 & 1 & 1 \\ 1 & 0 & 1\end{array}\right]$ over $\mathbb{Z}_{2}$...
5 answers
Fndom work was 22 6 miles ad the slanderd cavlation wes 6 / miles Asgume Ie populatlon MuLAI sampla ol Iive puople iha metn divirig distance Iouied and usu Iht | dsubuion ia ind Le Margu " eltor wnd coneiuc J 90 ccnliderice Inierval ior the populalion mean Iniarpret Ihe resulisbdenuty Ihe margin(Round(o one dedral pace: nedrd |Elx4Inx Wta cdorino Intncic CnecAmt
Fndom work was 22 6 miles ad the slanderd cavlation wes 6 / miles Asgume Ie populatlon MuLAI sampla ol Iive puople iha metn divirig distance Iouied and usu Iht | dsubuion ia ind Le Margu " eltor wnd coneiuc J 90 ccnliderice Inierval ior the populalion mean Iniarpret Ihe resulis bdenuty Ihe m...
4 answers
For the histogram on the right determine whether the mean is greater than; less than, approximately equa to the median Justify your answerWhich of the following is correct?x=Mbecause the histogram is skewed right x>M because the histogram is skewed X<M because the histogram is skewed leftx=Mbecause the histogram is symmetric x>M because the histogram is skewed right X<M because the histogram is symmetric
For the histogram on the right determine whether the mean is greater than; less than, approximately equa to the median Justify your answer Which of the following is correct? x=Mbecause the histogram is skewed right x>M because the histogram is skewed X<M because the histogram is skewed left x=...
5 answers
Give some chemical reactions for ethanol..
Give some chemical reactions for ethanol.....
5 answers
Write parametric equations for the circular path above beginning at the point (9, 0) and proceeding once around the circle in the counterclockwise direction. Use parameter- on the interval <t < 2.I(4)ult)Write parametric equations for the circular path above, beginning at the point (0; 9) and procceding once around the clrcle in the clockwise direction_ Use parameter on the interval 0 < <2 .r(l)V(l)
Write parametric equations for the circular path above beginning at the point (9, 0) and proceeding once around the circle in the counterclockwise direction. Use parameter- on the interval <t < 2. I(4) ult) Write parametric equations for the circular path above, beginning at the point (0; 9) a...
5 answers
-2 0 3 compute AB and BA, if possible. 4 -4 4If A = 80 and B0 -4
-2 0 3 compute AB and BA, if possible. 4 -4 4 If A = 8 0 and B 0 -4...
5 answers
Question 30/ 5 ptsUse any method to find the volume of the solid that results from rotating the region specified below around the indicated line:y = ~c2 + 6ry = 0; about the x 6xtSRound your answer to three decimal places
Question 3 0/ 5 pts Use any method to find the volume of the solid that results from rotating the region specified below around the indicated line: y = ~c2 + 6r y = 0; about the x 6xtS Round your answer to three decimal places...
5 answers
Find the exact value_ Write your answer as an integer or single fraction in simplest form_ Rationalize your denominator, if necessarySin arcsinarccos
Find the exact value_ Write your answer as an integer or single fraction in simplest form_ Rationalize your denominator, if necessary Sin arcsin arccos...
5 answers
1. Find the interval of convergence for the following series:()(x_3) a_ n=0 6"2n+]bnln=1x2 x4 X C. 1+ - + + +. 4 16 36
1. Find the interval of convergence for the following series: ()(x_3) a_ n=0 6" 2n+] b nl n=1 x2 x4 X C. 1+ - + + +. 4 16 36...
5 answers
10 pts (b) With the aid of simple diagrams, explain the difference between the Dispersion of light and the Diffraction of light;
10 pts (b) With the aid of simple diagrams, explain the difference between the Dispersion of light and the Diffraction of light;...

-- 0.019558--