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RNA RMRP and reverse transcription real time PCR howmeasuring it ???...

Question

RNA RMRP and reverse transcription real time PCR howmeasuring it ???

RNA RMRP and reverse transcription real time PCR how measuring it ???



Answers

What is the difference between reverse transcriptase PCR (RT-PCR) and real-time quantitative PCR (qPCR)?

Now regular PCR or eliminates chain reaction was already pretty cool. What to me to be released? How could we make this even more interesting or scientifically useful? Well, we go from just a regular PC off which has remember those three steps that d maturation there kneeling and the extension. And then we take it into rial time. PCR Now remember PCR opened the train reaction allows us to go from one being a friend and again, we always say one, but you're never one. Isolate just one Dean assuring. But she takes you from one in quotations trying to millions a point billions. No, we know that was broken down to three steps. You had to be nature. That's a Neil and you have to extend. And each day, three steps. A pretty easy to remember, because what happens is thanks for the pretty to me cool or easy to follow. When you d nature, you take a double stranded DNA, get it hot, and it makes to single friend Deena's. They'll tell you a Neil Dustin Science. They have some climber to stop opposing ends up, and this stores the process of allowing us to fail. Do you know, Blue Nice coming in and give us two new friends. Now, what makes real time PCR so useful? Is it alone the way we can actually have approved? So someone nucleotides, for example, could be attached to floor for and I write that out, but a floor for based, it just means in like the floor gives it away floor, folks, your maga presence so you can attach and make sure SP a floor full probably got. You can attach a floor full excited and lights up so it makes it tells you just how specific or just how many new DNA sequence since you have So we know it usually moving billions. But what we don't know is quite high meaning until we use real time PCR, which now in tell us just how many friends were made.

So replication versus transcription together. This time we're gonna talk about this speed. We know that replication synthesizes with Dina pulling race and very quickly. So this happens quickly and transcription with article in race appears to happen a little bit more slowly. We're going to recall that, uh, replication is genome wide. So the entire genome let's be replicated in order one toe when cells divide and when they grow. So this is why it happens a lot more quickly because no, just in humans with 46 chromosomes, this, um, is extremely, extremely a lot of information to replicate. And because of the size of this speed is relatively key and in transcription. This is only one gene at a time, so this process can afford to go a little bit more slowly and because it's transcribing something eventually to go into protein, it is beneficial for the cell to have checkpoints in place and things that keep the nuclear tides in a certain order and prevent any mutations for eventual protein. Beat me. Um, but the key here again is the size of the information we're going to transcribe or replicate cousin Gino wide replications. So much information it needs to be happy quickly

Explanation for the question. Yeah. Increasing. Okay. The error. Yeah. Okay. Dread up. Okay. Uh huh. Mhm transcription. Yeah. In crisis. Uh huh. The mutation. Mhm. Wow. Red dead. Yeah. Yeah. Province. The virus. Mhm from completing its life cycle in the hospital. Wow.

Although fascinated concept when we're talking about are any I I'm going to try to keep, I guess. Go away from trying to define this process with too much rigor on because that would take much longer than 23 minutes. Destiny did and stay. We'll try. I'm going to try to give you a liver brief definition. So, Arnie I or in a interference is the process in which are in a can actually inhibit gene expression. So we have Arnie now. It basically interrupts the gene expression by binding what it was called our ice SC complex against all the steps here and is very, very interesting. But it's also a very, very alone, so our SC complexes toe or in a induced complex, and this is a valuable research to because it is great at Silas and a gene.


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