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How does CRISPR/Cas9 work ( in detail by steps ) ?...

Question

How does CRISPR/Cas9 work ( in detail by steps ) ?

How does CRISPR/Cas9 work ( in detail by steps ) ?



Answers

How do siRNAs work?

I'll do micro ironies work. These are M I. R. Lays on the's are small pieces of irony. They are hairpins 22 22 nucleotides long, and they binds to Emma really on by binding to Enron A. They prevent it from being translated so effectively based silence that gene.

To answer this question, we first have to identify what restriction Endo new places are. So you're restriction and their nucleus is going to be an enzyme that recognizes a specific DNA sequence, and it will thereby cleave the DNA at that particular DNA sequence. When we say Cleaves the DNA, we mean that it cuts the DNA at that Ah, particular sequence. So it will essentially recognize a particular region of DNA, and this is going to be a very short sequence. So perhaps it might be like six or eight base pairs law or eight nucleotides long, and it will recognize the sequence, and it will basically bind to that sequence and catalyze the hydraulics ISS of the fossil digester bonds of the DNA backbone. So how will it cut the DNA to the hydraulic sis through the hydraulic sis of the phosphor O di Ester bonds of the DNA backbone? So this means that it will cut this DNA sequence by basically doing the opposite of how DNA on nuclear ties will pull. Memorize. So polarization is going to involve the condensation reaction of the addition of each particular nuclear tied to this growing Polly nucleotide chain whereas here, we're using water molecules to cut our poly nuclear tie chain or our DNA, thereby breaking those fossil disaster bonds and forming a cut in this DNA.

Let us talk about DNA diaries. Do you need a guide? Is is it Doughboy? Somebody's, which is found in back Dios is that introduces negative super coils into the detainee. This Dele Diaries is a drum wish and a glass do DuPuy Somebody's, which acts by looping the DNA around itself, which gives the negative super coy's to the dealing. Then, after this super coiling happens, it cuts both. This trends off the detainee on one part of the loop like one terminal off the loop and allows the other terminal do join the first dominant. So it utilizes energy toe got and based from the hydrolyzed sis off ADP on. Then it rejoins the Dina ins on again. After the rejoining, it releases it. That's why, in the beginning, I said it gives negative super coil.

In this example, they just want us to briefly explain the steps through its sciences applied. So the first thing we're gonna do is we're gonna go out in the world we're going to make an observation, right? We're going to see some sort of natural phenomenon that sparked our curiosity. We are then going to ask ourselves a question, right? Typically that question is going to be why does this happen? How does this happen? Um you know, something along those lines. Then before any testing or anything like that, we're just going to make a guess. You know, we're just going to form an ipod hypothesis. All right. It's just I guess so. I just think this happens because of this. All right. And then after that I'm going to make a prediction, Right? So typically the prediction is going to be something like I feel like if I change this variable that will cause it to act in this way. Right? So, I'm making my prediction. Um I'm then going to set up a test in order to test my hypothesis. To test my prediction. Right? So that's a very key point. When you do for your hypothesis, you want to make sure that it's testable, right? If I were to say that this happens because fairies do it, you know, we can't really at least right now we can't really test for ferries. So that's it's not really a it's not a strong hypothesis. Right? It's not a very good hypothesis. But anyway, we set up our test and we test our prediction. Right? So two things can happen. Write your prediction was correct or your prediction was right. Both are still good. Right? We learned from both. But if your prediction was correct, you can either creating another test maybe a more in depth test to solidify your hypothesis and if it was wrong, that's okay. You say maybe why it was wrong? Was your test not accurate enough? Um, Was your prediction bad? You know, you would go back either way, You would iterate the process, right? So you go back, maybe change your hypothesis, make a new prediction, create a new test and just keep going through those steps until you feel like you actually figured out why this phenomenon acted the way it did.


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