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Use Microsoft Excel software to create a data table. The table will look similar to the one shown below_Table 4. Data for the SDS-PAGE Standard Curve Standard Migra...

Question

Use Microsoft Excel software to create a data table. The table will look similar to the one shown below_Table 4. Data for the SDS-PAGE Standard Curve Standard Migration Rf Molecular Distance (mm) Mass (kDa) 250.000 2 150.000 100.000 75.000 1 50. 000 37.000 25.000 8 20.000 15.000 10 10.000Log Molecular MassADH

Use Microsoft Excel software to create a data table. The table will look similar to the one shown below_ Table 4. Data for the SDS-PAGE Standard Curve Standard Migration Rf Molecular Distance (mm) Mass (kDa) 250.000 2 150.000 100.000 75.000 1 50. 000 37.000 25.000 8 20.000 15.000 10 10.000 Log Molecular Mass ADH



Answers

The accompanying figure is from an electrophoresis experiment using SDS-PAGE. The left lane has the following standards: bovine serum albumin (MW 66,000 ), ovalbumin (MW 45,000 ), glyceraldehyde 3 -phosphate dehydrogenase $(\mathrm{MW} 36,000),$ car bonic anhydrase (MW 24,000 ), and trypsinogen (MW 20,000 ). The right lane is an unknown. Calculate the MW of the unknown.

Okay. So the method is jailed filtration which does this on the basis off size. Which means larger proteins will elude first due to their masses. Yeah. Okay. So we need to applaud june filtration of human cytochrome see and bacterial fage G seven on any polly Marie's. Okay, so this we are doing it from table 5.1. Okay. The plot is going to look something like this. This is going to be illusion volume And this is going to be absorbent at 2 80 nanometers and you would have something like this. Okay. And this is going to be all shaded. I mean it's all colored and this is back to you. Fage D seven are any preliminaries and the next one is going to be all red and this is going to be human side two. True. I see. Okay. Will we go into the next part as DS page. Okay. As your space also selling also separate on the basis of mosques. So sts bynes two proteins and all rooms. Yeah, entrance sick charge, resenting insane mass to charge ratio for all of the analyzed proteins. Okay, so similarly smaller proteins move fast. So in this case faster. Okay, so how you will do this is that straw. This is 99 one. This is 12. You would have won something like this because this is smaller than mosque. So this is back to you age 37 RNA polymerase and then you would have humans. Itis. So some very huge like this. I just want to fill it up. Okay, so this is how it is. And just for understanding this is the migration. Okay?

Question Number 10 is an interesting question that allows you to interpret separation data in order to determine the structure of the protein. When a protein is separated without being fragmented by size exclusion chromatography, it generates data that suggests a mass of 400 kill a Dalton's. Then, when it is broken into pieces using SDS, which causes it to unfold into three different constituents having a mass of 180 kill adult, 160 kill adulterants and 60 kill adult nuns. And when we some those up, we get our 400 Kila Dalton's. However, when we add STS and die die, 03 at all, we end up getting these three fragments 160 90 and 60. So it appears that the addition of Die Thigh 03 a tall has caused the 182 fragment further into to 90 pieces. So there appears to be four subunits to this protein. We've got the 160 and we've got to nineties, and then we've got a 16. The two nineties appeared to be bonded to each other and with just the STS are likely to be have there, um, die one or more die sulfide bonds holding the pieces together be broken with the addition of the Dai Kaio three at all.

And so far they got in here. The 14 have to 40 daddy and do get the political plates. The 230 polyp, uptight, is a test non violently to the 100 carry politics died mhm 24 mm, a protein.

Problem. 17. Stem and leaf on the plot. Draw a vertical line, please. The digits on 10. So we're the same. It's 6789 Edley is 78 at 35569 0023557 78 And neither 1112455 on the matter. This is the stem and develop. So the matter distribution is skewed to the left because most off the data various live to the bottom. Off the graph. Most off the exam schools appeared to lie between 80 at 99 most of the data very care as 10 8 and stand mine in the graph.


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