All right, We're being asked to determine, um, which of the fooling choices is correct regarding, uh, d n a sequencing methods and their correct description. So let's start with the 1st 1 The chain termination method. The change from an F A termination method is a method where you want to, uh, replica. You want to see once a certain target D n A. And you put that target D n a in a flask. Try and draw last here. All right, you put that DNA in a flask along with some nucleotides, right click. I try phosphates, and along with some dye, the oxy nucleotide try phosphates. Okay? And they go all in there with the DNA and as the DNA is growing. So let me go over here now. All right, So this is our target, D n a. And you. You put a primer on the DNA and you start replicating with Vienna after the primer. Now, as the DNA starts growing, um, it's gonna get incorporated regular nuclear ties in there, but sometimes just by chance alone since they are in solution, the diet Dina di di Oxy nucleotide will get incorporated into the chain and that can happen either towards the beginning, towards the end or somewhere in the middle. And the addition off a dye, the oxy nucleotide to the chain terminates replication. It stops replication, right, which is why it's called chain termination Method. So basically, you start producing a bunch of different chains and just by chance alone, those chains are going toe. Stop reproducing or not not read this. We are going to stop being replicated at some point in time just by the addition off the dive, the Oxy nuclear tie. And then you sequence all of those chains and you can then see that. Okay, if the replicated thing if the replicated DNA is one nucleotide long and the first dd NTP that was incorporated is a G than the first letter is a G and then you say OK, if the replicated d n a stretches two nucleotides long and the second letter is a tea, well, then we now that we have g and T for the first right for the first and second. So now that you know the first and second you pick the fragment with three nucleotides law and that one is also a tea. Okay, so we have G TT, and that's how you build the entire sequence. And this is a little bit time consuming, but this is how we first used to um uh, determine the sequence off D N A. Now let's move into shotgun sequencing. So shotgun sequencing basically does multiple, uh, change termination methods at once. So instead of just taking one instead of just taking one fragment of the n A and making a bunch of different length replications of that and then sequencing, it's one of those. What you do instead is you take the D N A. You break it into a bunch of different fragments and then you sequence every single one of those fragments by the corporation of the D N tp here. So you basically determine what that last Ah ah nucleotide was here. And once you have all the different fragments, you can align them by finding sequences of similarity. So you're like, OK, this is an overlap. So that means that the end off this fragment must match to the beginning of that fragment. So you incorporate that into the sequence and at the end you have the sequence off the large in the molecule. It's called shotgun sequences because you basically do a bunch of the same time, not one at a time. And a next gen sequencing. Um, I don't have a good graphic for this, but it is basically an automated process that performs thousands of these guys simultaneously. So it's an automated process with, like a robotic arms. Everybody pipettes and the DNA sequences get, uh, get determined. And then those sequences are a line in the computer, and the computer spits out one long D n a sequence. So that's how that works. Now let's look at our answer choices. Choice. A change termination method is automated sequences are well, that is wrong right away because in change termination, which was this first guy over here? This is how we used to do it. Back in the day, there was no automated sequence. There's here. Choice Be change Termination A Change Termination Method Inc of DD entities during d n. A. Replication. Yes, shotgun sequencing. Cutting DNA into random fragments sequence using transformation and assembling overlapping sequences. Yes, that is correct. Choice. The next Ah thing in choice B is next generation sequencing automated sequences. Sequencers are used to generate sequences of short fragments, and that is correct because it is automated. Choice E is wrong because, well, actually, let me read it to you. And then so choice. A sea change Termination Method Inc off dd ntp during the DNA replication. Yes, that is correct. Shotgun sequencing. Automated sequences are used. No in shotgun sequences, you don't have automated sequence. There's so choice, he's wrong and Choice d that's wrong for the same reason it's choice A because it says that automated sequencers are used in turning the chain termination method. So the only, uh, answer here that has all of the correct descriptions is Choice B.