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MacConkey agar is selective because it: it contains blood and hemolytic organisms can be identified inhibits gram 1-negative organisms it contains an indicator t0 i...

Question

MacConkey agar is selective because it: it contains blood and hemolytic organisms can be identified inhibits gram 1-negative organisms it contains an indicator t0 identify lactose- ~fermenting organisms inhibits gram-positive organisms

MacConkey agar is selective because it: it contains blood and hemolytic organisms can be identified inhibits gram 1-negative organisms it contains an indicator t0 identify lactose- ~fermenting organisms inhibits gram-positive organisms



Answers

Blood agar contains many unspecified nutrients, supports the growth of a large number of bacteria, and allows differentiation of bacteria according to hemolysis (breakdown of blood). The medium is _________ and _____________.

For this question, it's important to know the difference between selective media's differential media's and of course complex media's. So starting at the top, a selective media is one that's going to select for a certain type of organism. So here, the important thing is we're going to have growth of some organisms that we want and we're going to have no growth of the organisms that we do not want. So here a selective media is one that chooses for the growth of one organism or for the non growth of others. This is opposed to a differential media where each organism will be able to grow. But some sort of quality is going to be different between the two. This could be something such as pigment or sort of a feature displayed by it. So on a McConkey plate or in this case in months and methylene blue plate, we are going to see some sort of coloration difference between different types of organism and that will help us tell the difference between the two and make a conclusion about what the organism is. Lastly for a complex plate or medium. This is one where we don't know the exact concentration of the contents of the plate. The reason we use these is because it's easier in some circumstances to make a plate from a substance where we don't know the exact contents. It sounds complicated, but this could be something like using red blood cells in order to give nutrients to the plate. So if you've heard of an S. B. A. Plate or a sheep blood auger, this is one where we use sheep blood cells in order to have that nutrient source available for the organisms we are growing. We do not measure the amount of amino acids, proteins or nutrients supplied by those red blood cells, but we do know that each of those substances aren't going to be there due to the qualities of a red blood cell. This is different from, say, a simple medium where we're going to measure out each of the contents such as the amino acids, the nutrients and other certain quantities to make the actual medium itself. So here complex tends to be a little bit more easier because it's going to supply all the nutrients with the addition of one single substance rather than through the measurement of multiple nutrients, making sure we have each nutrients that's required for the plate and through all that complex process. So here for this question we are talking about an M. B. Media or an Essene methylene blue. And here the important things to note are that we are going to select for a gram negative organisms and that we can get a metallic sheen from certain organisms we are trying to grow. So here, for the first part, since we are selecting for gram negative bacteria, only our grand positive are going to show no growth. So this is going to have a selective effect to it because we are selecting only for the gram negative by excluding nutrients or by adding contents that are going to prevent the growth of these gram positive organisms. For this metallic sheen aspect though this is going to serve as a differential aspect to it because we are adding a quality to certain organisms, we will see this sheen whereas in others we will not we are not selecting for the growth or non growth of the different organisms by whether they show the metallic sheen or not. But rather we are being able to tell the difference or differentiate between the two based on this visual feature. So here the E. M. B plate is going to be both selective and differential. And in order to make this, we're going to include some complex ingredients as well, such as red blood cells for nutrients where we're not going to exactly measure out each of the contents and make sure they are steady and equivalent and amount. So here an E. M. B plate, which is often used to identify certain strains of E. Coli, is going to be selective, differential and complex. So here the best answer choice for this question is going to be D.

For this question, we are looking at a sheep blood auger culture plate. So here we're looking for some terms that describe the media being used. So here we have some terms we can go over we have selective media that we can use. We have differential and we also have complex and simple. So here a sheep blood auger is going to be one where we take a gel like substance typically from algae and we're going to add red blood cells typically from sheep. And here this edition of red blood cells is important to what makes this auger special. Either one of these or multiple of these qualities. So what's important about this red blood cell edition is that we're going to get the proper nutrients we need to grow organisms. But what we don't know is the certain concentrations of certain ions such as sodium or chloride and we don't know the certain contents or concentrations of specific amino acids. So some qualities are going to be unknown based on this edition of these red blood cells. So here, what we used to define our media that contain these lesser known contents is going to be a complex media. So here the difference between a complex and a simple media is knowing these concentration rate. So a simple media, we're going to directly add exact quantities of, say amino acids and ions. So here the media is going to be complex rather than simple. What we're also told about this media is that we're able to differentiate between different organisms based on hemodialysis. So this is a way to tell the difference based on whether we have incomplete licensing of the red blood cells. We have complete licensing in a large clearing area around the Seth bacterial cells. Or there is no Hamal Asus at all. So these are either alpha beta or gamma Hamal icis. So here this is going to be a differential media quality because we are able to tell the difference or differentiate between different organisms. This is opposed to a selective media which is going to show either growth of certain organisms or it's going to prevent the growth of others. So it's selecting for the growth or no growth of organism. And this is not selected by on a sheep blood agar plate and it's going to grow most organisms equally. So here a sheep blood auger is going to be both differential based on Hamal icis and complex because we add this uncertain quantity of ions and amino acids through the addition of red blood cells. So here the film, the blank answers should be differential and complex.

So looking at different types of vega this time we're looking at Ragusa to reduce, Okay, we know the ph is 5.2 so it's acidic and that discourages microorganisms. But anything that does go on their looks quite similar. What can we say about this medium? First of all? Is it going to be a medium where it is chemically defined? That is we know every molecule in it, every compound. Where is it going to be complex where we cannot have a precise composition, what we're told. But it is derived from yeast and containing yeast extract means that it's going to be complex. It's complex if you have an Eggo but contains some kind of extract of another organism like yeast or like blood for example, it's going to be complex because we don't have a perfect composition of it. Next we can see that the colonies will look similar, but only some microorganisms can grow on it and because it restricts for growth of unwanted microorganisms, that means it is selective. So it's a complex, selective medium.

For this question, We're talking about the organism Haemophilus influenza. And we are told that it requires a special auger or nutrient Wagner to grow on called a chocolate auger. So the reason we call it a chocolate auger is because it has this brownish appearance and it is very nutrient rich. So how we repair this is we take a regular sheep would augur, we're going to apply heat and it's going to allow for the release of the red blood cell contents and the oxidation of some of those sources. So here we're going to get that brown coloration for which we call it chocolate. So here, the important part is that we are realizing those red blood cells and this is going to release their contents onto the Petri dish, allowing it for organisms such as Hemophilus influenza to take them up better. And this is because this organism requires special nutrients called V and X factors. And these are going to be found as part of the poor fear imagines or the hemoglobin components of your red blood cells. So here because we require these special nutrients and they're not going to grow on our normal plates, these are what we call those difficult to grow organism where unless we supply this extra nutrients or the specialist environment, they're not going to grow. So rather than calling them a difficult organism, we use a more scientific term called fastidious, just meaning they're more difficult to grow and require those special requirements. So here you should pick answer choice. D.


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